Perfluorosulfonic acid polymer-coated indicator electrodes

Details Perfluorosulfonic acid polymer-coated indicator electrodes Perfluorosulfonic acid polymer-coated indicator electrodes

1985-01-29

1986-08-05

Niebling, John F.

Chemistry: electrical and wave energy

Apparatus

Electrolytic

204415, 435288, 435291, C12Q 154

Patent

active

046041826

ABSTRACT:
A method is described for the amperometric determination of the concentration in a biological sample of a constituent which is a substrate for an oxidase enzyme by (1) catalyzing the substrate with the enzyme, thereby consuming O.sub.2 and producing H.sub.2 O.sub.2 and (2) measuring the O.sub.2 consumption or H.sub.2 O.sub.2 production by means of an inert metal indicator electrode intimately coated with a thin film of a perfluorosulfonic acid polymer.
A reaction chamber is described which provides means for contacting the sample with the enzyme and means for making amperometric measurements utilizing inert metal indicator electrodes coated with a thin film of a perfluorosulfonic acid polymer.

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