Percoll and sugar self-forming density gradient

Details Percoll and sugar self-forming density gradient Percoll and sugar self-forming density gradient

1998-04-23

1999-11-16

Brunsman, David

Sugar, starch, and carbohydrates

Products

Miscellaneous

422 72, 422101, 494 27, C13F 300, G01N 930, B04B 1510

Patent

active

059850370

ABSTRACT:
A stable self-forming density gradient is created by applying a centrifugal field to a solution that contains 27-33% Percoll (v/v) and 36-44% sugar (w/w). This density gradient is stable for several hours, allowing separation of low sedimentation particles, such as subcellular particles.

REFERENCES:
patent: 4480038 (1984-10-01), Cheng
patent: 5171539 (1992-12-01), Coombs
CA 101:187340, Nagy et al, "Rapid Preparation of . . . ", 1984.
CA 107:130325, Symons et al, "Isolation of highly purified . . . ", 1987.
CA 106:47644, Jadot et al, "Intracellular pathway followed by . . . ", 1986 .

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