Peracetic acid sterilization of collagen or collagenous tissue

Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Inactivation or attenuation; producing viral subunits

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422 28, C12N 706, A61L 218

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054609623

ABSTRACT:
This invention is directed to the sterilization of collagen and collagenous tissue which is to be used for implantation, repair, or use in a mammalian host in the emerging field of tissue engineering. The sterilants are low concentration peracetic acid solutions in either neutral or high ionic strength that prevent or minimizes the swelling of collagen or collagenous tissue so that the sterilized tissue retains its structural integrity and bioremodelable properties.

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Wutzler, P. et al., "Suitability of Peracetic Acid for Sterilization of Media for Mycoplasma Cultures," Journal of Clinical Microbiology, Mar. 1975, pp. 246-249, vol. 1.
Von Versen, R. et al., "The Peracetic acid/low Pressure Cold Sterilization-A new method to Sterilize Corticocancellous Bone and Soft Tissue," Z. exp. Chir. Transplant. kunstl. Organe 22 (1989) pp. 18-21.
Block, Seymour, "Peracetic Acid," Disinfection, Sterilization and Preservation, 4th edition, pp. 172-179, 1993.
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