Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Isomerase
Patent
1990-07-19
1994-02-08
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Isomerase
43525231, 4353201, 536 232, C12N 990, C12N 1561, C12N 1570
Patent
active
052847624
ABSTRACT:
A yeast PPIase characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro stands for L-proline), (2) exhibiting a single molecular weight of about 17,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 6.2 in the isoelectric focusing, and (4) inhibited by CsA;
an E. coli PPIase-.beta. characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 20,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 5.0 in the isoelectric focusing, and (4) no being inhibited by CsA;
an E. coli PPIase-.alpha. characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 22,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 9.7 in the isoelectric focusing, and (4) not being inhibited by CsA.
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Hayano Toshiya
Katou Setsuko
Maki Noboru
Suzuki Masanori
Takahashi Nobuhiro
Meller Michael N.
Moore William W.
Tonen Corporation
Wax Robert A.
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