Peptides with enhanced stability to protease degradation

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues

Reexamination Certificate

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C530S327000, C514S04400A, C435S006120

Reexamination Certificate

active

10252773

ABSTRACT:
Disclosed are peptides that have enhanced stability against plant proteases and are useful in the control of plant diseases. The peptides also have the ability to protect other peptides, polypeptides or proteins from degradation by proteases of plant, fungal, viral, bacterial, insect or other origin. DNA encoding the peptides of the present invention can be co-expressed with other DNA encoding exogenous peptides in transgenic plants as a method for protecting foreign peptides from degradation by proteases. Also disclosed are nucleic acid sequences, microorganisms, plants, and compositions useful for the treatment of plant diseases.

REFERENCES:
patent: 5421839 (1995-06-01), Ulbrich et al.
patent: 5424395 (1995-06-01), Bascomb et al.
patent: 5519115 (1996-05-01), Mapelli et al.
patent: 5773696 (1998-06-01), Liang et al.
patent: 5824869 (1998-10-01), Broekaert et al.
patent: 5850025 (1998-12-01), Mirkov et al.
patent: 6015941 (2000-01-01), Rao
patent: 6538106 (2003-03-01), Fraser et al.
patent: WO 98/07745 (1998-02-01), None
Iwahori et al.,Synthesis of Reversed Magainin 2 Analogs Enhanced Antibacterial Activity, Biol. Pharm. Bull. 20(3):267-270 (1997).
Selsted et al.,Indolicidin, a Novel Bactericidal Tridecapeptide Amide from Neutrophils, J. Biol. Chem. 267(7):4292-4295 (1992).
Subbalakshmi et al.,Requirements for Antibacterial and Hemolytic Activities in the Bovine Neutrophil Derived 13-residue Peptide Indolicidin, FEBS Letters 395:48-52 (1996).
Subbalakshmi et al.,Interaction of Indolicidin, a 13-Residue Rich in Tryptophan and Proline and its Analogues with Model Membranes, J. Biosci. 23(1):9-13 (1998).
Van Abel et al.,Synthesis and Characterization of Indolicidin, a Tryptophan-rich Antimicrobial Peptide from Bovine Neutrophils, Int. J. Peptide Protein Res. 45:401-409 (1995).
Ladokhin et al.,Bilayer Interactions of Indolicidin, a Small Antimicrobial Peptide Rich in Tryptophan, Proline, and Basic Amino Acids, Biophysical Journal 72:794-805 (1997).
Owens et al.,A Single Amino Acid Substitution in the Antimicrobial Defense Protein Cecropin B is Associated with Diminished Degradation by Leaf Intercellular Fluid, MPMI 10(4):525-528 (1997).
Jaynes et al.,Increasing Bacterial Disease Resistance in Plants Utilizing Antibacterial Genes from Insects, BioEssays 6(6):263-270 (1987).
Lim et al.,Synthesis and Bioligical Characterization of Indolicidin Analogues, J. Biochem. Mol. Biol. 30(3):229-233 (1997).
Merrifield et al.,Retro and Retroenantio Analogs of Cecropin-Melittin HybridsProc. Natl. Acad. Sci. USA 92:3449-3453 (1995).
Mills et al.Evidence of the Breakdown of Cecropin B by Proteinases in the Intercellular Fluid of Peach Leaves, Plant Sci. 104:17-22 (1994).
Robinson, Jr. et al.,Anti-HIV-1 Activity of Indolicidin, an Antimicrobial Peptide from Neutrophils, J. Leukocyte Biol. 63:94-100 (1998).
Everett, N.P., Design of Antifungal Peptides for Agricultural Applications, Chapter 20, pp. 279-291 (1994).
Allefs et al.,Erwina Soft Rot Resistance of Potato Cultivars Transformed with a Gene Construct Coding for Antimicrobial Peptide Cecropin B is not Altered, Am. Potato J. 72:437-445 (1995).
Falla et al.,Mode of Action of the Antimicrobial Peptide Indolicidin, J. Bio. Chem. 271(32):19298-19303 (1994).
Falla et al.,Improved Activity of a Synthetic Indolicidin Analog, Antimicrobial Agents and Chemotherapy 41(4):771-775 (1997).
Florack et al.,Expression of Giant Silkmoth Cecropin B Genes in Tobacco, Transgenic Research 4:132-141 (1995).
Hancock, E.W. and Lehrer, R.,Cationic Peptides: A New Source of Antibiotics, Tibtech 16:82-88 (1998).
Piers et al.,Recombinant DNA Procedures for Producing Small Antimicrobial Cationic Peptides in Bacteria, Gene 134:7-13 (1993).
Enfors, S.,Control of in vivo Proteolysis in the Production of Recombinant Proteins, Tibtech 10:310-315 (1992).
Helmerhorst et al.,A Critical Comparision of the Hemolytic and Fungal Activities of Cationic Antimicrobial Peptides, FEBS Letters 449:105-116 (1999).
Peschel et al.,Inactivation of the dlt Operon in Staphylococcus aureus Confers Sensitivity to Defensins, Protegrins, and Other Antimicrobial Peptides, J. Bio. Chem. 274(13):8405-8410 (1999).
Rao, A.G.,Antimicrobial Peptides, MPMI 8(1):6-13 (1995).
Broekaert et al., Antimicrobial Peptides from Plants, Critical Reviews in Plant Sciences 16(3):297-323 (1997).
Mourgues et al.,Strategies to Improve Plant Resistance to Bacterial Diseases Through Genetic Engineering, Tibtech 16:203-210 (1998).
Mourgues et al.,Activity of Different Antibacterial Peptides on Erwinia amylovora Growth, and Evaluation of the Phytotoxicity and Stabulity of Cecropins, Plant Sci. 139:83-91 (1998).
De Bolle et al.,Antimicrobial Peptides from Mirabilis jalapa and Amaranthus caudatus: Expression, Processing, Localization and Biological Activity in Transgenic Tobacco, Plant Mol. Bio. 31:993-1008 (1996).
Cavallarin et al.,Cecropin A-Derived Peptides are Potent Inhibitors of Fungal Plant Pathogens, MPMI 11(3):218-227 (1998).
Powell et al.,Synthetic Antimicrobial Peptide Design, MPMI 8(5):792-794 (1995).
Chrispeels, M.J.,Sorting of Proteins in the Secretory System, Annu. Rev. Plant. Physiol. Plant Mol. Biol. 42:21-53 (1991).
P. Staubitz et al., Journal Of Peptide Science, 7:552-564 (2001).
Merrifield, et al., Proc. Natl. Acad. Sci. 92:3449-3453 (1995).
Ido, et al., Science 277:563-566 (1997).
Pinilla, et al., J. Mol. Biol. 283:1013-1025 (1998).
Vunnam, et al., J. Peptide Res. 51:38-44 (1998).
Zanetti, et al., FEBS 374:1-5 (1995).
Nagpal, et al., J. Biol. Chem. 274(33):23296-23304 (1999).
Gao, et al., J. Clin. Inv. 106(3):439-448 (2000).

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