Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-11-08
2000-09-12
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, C12Q 168, C12P 1934
Patent
active
06117632&
ABSTRACT:
A method of identifying a peptide which permits or facilitates the transport of an active agent through a human or animal tissue. A predetermined amount of phage from a random phage library or preselected phage library is plated unto or brought into contact with a first side, preferably the apical side, of a tissue sample or polarized tissue cell culture. At a predetermined time, the phage which is transported to a second side of the tissue opposite the first side, preferably the basolateral side, is harvested to select transported phage. This modified phage is amplified in a host. This cycle of events is repeated (using the transported phage produced in the most recent cycle) a predetermined number of times to obtain a selected phage library containing phage which can be transported from the first side to the second side. Lastly, the identity of at least one peptide coded by phage in the selected phage library is determined to identify a peptide which permits or facilitates the transport of an active agent through a human or animal tissue.
REFERENCES:
patent: 5672584 (1997-09-01), Borchardt et al.
Harbottle et al, Gene Therapy (1, Suppl. 2, 513), 1994.
Fong, S. et al., Scanning whole cells with Phage-Display libraries: Identification of Peptide Ligands that Modulate Cell Function, Drug Development Research, 33:64-70 (1994).
Stevenson, C.L.,et al., Permeability screen for synthetic peptide combinatorial libraries using CACO-2 cell monolayers and lc/ms/ms, Pharmaceutical Research, 12 (9), S94 (1995).
Pasqualini, R., and Ruosianinti, E., Organ targeting in vivo using phage display peptide libraries, Nature vol. 380, Mar. 28 (1996).
Barry, M.A., et al., Toward cell-targeting gene therapy vectors: Selection of cell-binding peptides from random peptide-presenting phage libraries, Nature Medicine, vol. 2, No. 3, Mar. (1996).
Russell, S.J., Peptide-displaying phages for targeting gene delivery?, Nature Medicine, vol. 2, No. 1, Mar. (1996).
Doyle, M.V., et al., The Utilization of Platelets and Whole Cells for the Selection of Peptides Ligands from Phage Display Libraries, Chapter 9:159-174, IN: Combination Libraries, Ed. Riccardo Cortese (Walter de Gruyter) 1996.
Nicosia, A., et al., Discovery of Disease-Specific Mimotopes by Screening Phage Libraries with Human Serum Samples, 9:145-157, (1996).
Anderson Kirsten A.
Campbell Eggerton A.
Elan Corporation, plc
LandOfFree
Peptides which enhance transport across tissues and methods of i does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Peptides which enhance transport across tissues and methods of i, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Peptides which enhance transport across tissues and methods of i will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-94405