Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 15 to 23 amino acid residues in defined sequence
Patent
1985-08-08
1987-03-03
Foelak, Morton
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
15 to 23 amino acid residues in defined sequence
530327, 530328, 530329, 530330, 530812, 530829, C07K 706, C07K 708, C07K 710, G01N 3700
Patent
active
046476547
ABSTRACT:
Monoclonal antibodies specific for the glucosylated N-terminal peptide residue in Hb A.sub.1c, a method for producing such antibodies, hybridoma cell lines secreting such antibodies and a method for their production, and immunoassay methods and reagent systems using such antibodies for the determination of Hb A.sub.1c in human blood samples. The monoclonal antibodies are secreted by hybridomas obtained from the fusion of a myeloma cell and a lymphocyte which has been taken from an animal, preferably a mouse, immunized with a synthetic peptide immunogen and which produces antibody specific for the glucosylated N-terminal peptide residue in Hb A.sub.1c. The synthetic peptide immunogen comprises an N-terminal glucosylated peptide residue having at least 2 amino acid units corresponding to the N-terminus of the beta-subunit of human hemoglobin and an immunogenic carrier to which the glucosylated peptide residue is linked. The immunoassay involves treatment of the blood sample to expose the glucosylated N-terminal peptide epitope and detection thereof by binding of the specific monoclonal antibody or a fragment thereof.
REFERENCES:
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patent: 4423034 (1983-12-01), Nakagawa et al.
patent: 4478744 (1984-10-01), Mezei et al.
Dixon, Biochem. J. (1972) 129, 203-208.
R. C. Boguslaski and T. M. Li "Homogeneous Immunoassays", 7, 401-414 (1982).
The Derwent Abstract of 86-119835/19 (DE 3 439 610) of Boehringer Mannheim GmbH.
Haigh Wallace
Knowles William J.
Marchesi Vincent T.
Foelak Morton
Molecular Diagnostics, Inc.
Nutter Nathan M.
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