Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues
Patent
1996-03-27
2000-12-12
Minnifield, Nita
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
4241841, 4242341, 4242421, 4241851, 424401, 514 44, 530324, 530325, 530326, 530327, 530328, 530329, 530330, A61K 3800, A61K 3170, A61K 3902, C07K 1700
Patent
active
061600873
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to peptides corresponding to fragments derived from the amino acid sequence of the 41 kD subunit protein constituting the fimbriae of Porphyomonas gingivalis, and their uses. More specifically, the invention relates to peptides acting as antigens which antigen-antibody react with antibodies against the 41 kD subunit protein, and uses of the peptides for detection of specific antibodies, etc. in the serum, saliva and gingival crevice fluid of patients with periodontal disease, and for prophylactic agents and treating agents of periodontal disease.
2. Description of Related Art
Periodontal diseases are classified into gingivitis and periodontitis, and further, periodontitis includes adult periodontitis, localized juvenile periodontitis, etc., but actually, 90% or more of periodontitis is occupied by adult periodontitis. These periodontal diseases are diseases including inflammations of gingiva, bleeding, drainage, formation of periodontal pockets, destruction of periodontal membranes, absorption of alveolar bones, and lability or loss of teeth. Various bacteria exist at the lesions of these periodontal diseases, and among them, Porphyomonas gingivalis is considered to be a main periodontopathic organism, and remarkable increase of the bacterium is observed at the lesions of periodontal diseases particularly adult periodontitis.
At present, methods for treating periodontal disease are not perfectly established, and it is considered to be the most important to find periodontal disease as early as possible, grasp its pathologic states accurately, and make appropriate treatments, and further, prophylaxis of periodontal disease by development of vaccines for periodontal disease is desired.
First, as to diagnosis of periodontal disease, a diagnostic method for finding periodontal disease as early as possible and grasping its pathologic state accurately has been expected, but actually, reliable diagnostic drugs for periodontal disease has not yet been developed. However, if ventured to be mentioned, as a means for diagnosing periodontal disease through paying attention to periodontopathic organisms, there are various methods for knowing the presence or number of these periodontopathic organisms. Further, there have been proposed methods to assay a specific antibody against a periodontopathic organism and utilize the result for diagnosis of periodontal disease. Still further, methods have been proposed comprising assaying an inflammatory product in the gingival crevice fluid of a patient with periodontal disease.
There are various methods for knowing the presence or number of these periodontopathic organisms. For example, it was conducted to culture periodontopathic organisms in the dental plaques, gingival crevice fluid, saliva, etc. of a patient with periodontal disease in a blood agar medium under an anaerobic condition, and investigate detailed biochemical properties of the resultant various colonies, and thereby detect the periodontopathic organisms (Loesche, W. J., Syed, S. A., Schmidt, E. and Morrison, E. C.: J. Periodont. 56, 447-456, 1985, etc.).
There has been conducted Gram staining, under microscopic observation, of periodontopathic organisms in dental plaques, gingival crevice fluid, saliva, etc. of a patient with periodontal diseases, or there has been made an examination under a dark-field microscope (Listgarten, M. A. and Levin, S.: J. Clin. Periodontol. 8, 122-138, 1981, etc.). There has also been carried out the detection of a periodonto-pathic organism by combining an antibody against it with a fluorescent dye (Zambon, J. J., Bochacki, V. and Genco, R. J.: Oral Microbiol. Immunol. 1, 39-44, 1986).
Further, it has been conducted to detect periodontopathic organisms in the dental plaques, gingival crevice fluid, saliva, etc. of a patient with a periodontal disease, according to an immunological method such as enzyme-linked immunosorbent assay (ELISA method), using antibodies against the respective periodontopathic o
REFERENCES:
patent: 4661350 (1987-04-01), Tsurumizu et al.
patent: 4689221 (1987-08-01), Kiyoshige et al.
patent: 5212059 (1993-05-01), Schwartz et al.
patent: 5310542 (1994-05-01), Au et al.
patent: 5334503 (1994-08-01), Snyder et al.
patent: 5348733 (1994-09-01), Morishima et al.
patent: 5432055 (1995-07-01), Evans et al.
patent: 5494672 (1996-02-01), Hodges et al.
patent: 5536497 (1996-07-01), Evans et al.
patent: 5830710 (1998-11-01), Progulske-Fox et al.
patent: 5948636 (1999-09-01), Mori et al.
patent: 6017532 (2000-01-01), Travis et al.
Dashper et al, Australian Dental Journal, 43/2:99-104, 1998.
Nakamura et al, FEMS Microbiol. Lett., 175:267-72, 1999.
Weinberg et al, Infection & Immunity, 65/1:313-316, 1997.
Choi et al, Infection & Immunity, 66/1:391-393, 1998.
Ogawa et al, FEMS Immunol & Med. Microbiol 11:247-56, 1995.
Deslauriers et al, Inf & Imm. 64(2):434-440, 1996.
Hamada et al, Inf & Imm. 64(11):4788-4794, 1996.
Nakayama et al, J. Bacteriol, 178(10):2818-2824, 1996.
Chandad et al, Inf & Imm. 63(12):4755-4763, 1995.
Amano et al, Inf & Imm. 62(8):3372-3380, 1994.
Ogawa et al, Vaccine, 15(2):230-36, 1997.
Nagata et al, Inf & Imm. 65(2):422-427, 1997.
Kawata et al, Inf & Imm. 65(2):815-817, 1997.
Weinberg et al, Inf & Imm. 65(1):313-316, 1997.
Lee et al, Inf & Imm., 59(1):383-389, 1991.
Ogawa et al, FEMS Microbiol Letters, 120:23-30, 1994.
Malek et al, J. Bacteriol, 176(4):1052-1059, 1994.
Evans et al Inf & Imm, 60(7):2926-2935, 1992.
Fujiwara et al, BBRC, 197(1):241-247, 1993.
Houghton et al, Vaccines 86 pp 21-25, 1986.
Bowie et al, Science, 247:1306-1310, 1990.
Bixler et al, Synthetic Vaccines vol. 1:39-71, 1987.
Muhammad et al, Pakistan Vet. J. 16(3):119-121, 1996.
Sharma et al, Appl. & Environ. Microbiol, 62(11):3933-38, 1996.
Klausen et al, Oral Microbiol. Immunol., 6:193-201, 1991.
Ogawa et al, Oral Microbiol. Immunol., 6:332-340, 1991.
Ogawa et al, Vaccine, 15/2: 230-236, 1997.
Sharma et al, Appl. & Environ. Microbiol 62/11:3933-3938, Nov. 1996.
Hamada et al Microbiol Immunol 38/12: 921-930, 1994.
Ogawa et al, J. Med. Microbiol 40:397-402, 1994.
Evans et al, In: Molecular Pathogenesis of pp267-278 Periodontal Disease. Editors Genco et al, 1994.
Ogawa, J. Med. Microbiol, 41:349-358, 1994.
Ogawa et al, Vaccine 15/15:1598-1605, 1997.
W.J. Loesche et al., "Bacterial Profiles of Subgingival Plaques in Periodontitis", J. Periodont, 56, 447-456, 1985.
M.A. Listgarten et al., "Positive correlation between the proportions of subgingival spirochetes and motile bacteria and susceptibility of human subjects to periodontal deterioration", J. Clin. Periodontol, 8, 122-138, 1981.
J.J. Zambon et al., "Immunological assays for putative periodontal pathogens", Oral Microbiol. Immunol. 1, 39-44, 1986.
W.J. Loesche et al., "The identification of bacteria associated with periodontal disease and dental caries by enzymatic methods", Oral Microbiol. Immunol. 1, 65-70, 1986.
C. Mouton et al., "Serum Antibodies to Oral Bacteroides asaccharolyticus (Bacteroides gingivais): Relationship to age and Periodontal Disease", Infection and Immunity, 31, 182-192, 1981.
T. Ogawa et al., "Bacteroides-specific IgG and IgA subclass antibody-secreting cells isolated from chronically inflamed gingival tissues", Clin. Exp. Immunol. 76, 103-110, 1989.
Ogawa et al., "Immunobiological activities of synthetic peptide segments of fimbrial protein from Porphyromonas Gingivalis", BBRC, 180, No. 3, 1335-1341, 1991.
Evans et al., "Immunization with fimbrial protein and peptide protects against Porphyromonas Gingivalis-induced periodontal tissue destruction", Adv. Exp. Med. Biol. 327, 255-262, 1992.
Lee et al. "Synthetic Peptides Analogous to the Fimbrillin Sequence Inhibit Adherence of Porphyromonas gingivalis", Infection and Immunity, vol. 60, No. 1, pp. 1662-1670, Apr. 1992.
Ogawa et al., "Hemagglutinating and Chemotactic Properties of Synthetic Peptide Segments of Fimbrial Protein from Porphyromonas gingivalis", Infection and Immunity, vol. 62, No. 8, pp. 3305-3310, Aug. 1994.
Dickinson
Kyowa Hakko Kogyo Co, Ltd.
Kyowa Medex Co., Ltd.
Meito Sangyo Kabushiki Kaisha
Minnifield Nita
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