Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 8 to 10 amino acid residues in defined sequence
Patent
1992-06-17
1995-07-18
Hill, Jr., Robert J.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
8 to 10 amino acid residues in defined sequence
530329, 530330, 530331, 424 851, C07K 706, A61K 3702
Patent
active
054342473
ABSTRACT:
The present disclosure relates to a new lymphokine molecule termed Monocyte Cytotoxicity Inducing Factor (MCF), to MCF peptides. The disclosure further relates to the development of novel Sezary cell hybridomas which secrete MCF, to the purification of MCF and its constituent polypeptides and to the identification of small peptides with MCF activity. Sezary OKT4+ lymphocytes were fused to CEM.8aza.sup.r.C, an HGPRTase lacking clone of CEM, to generate hybrid cells, certain of which produced soluble mediators of human monocyte cytotoxicity. A single sezary hybrid clone, FtF3, produced a novel monocyte cytotoxicity inducing factor found to be distinct from IFN.gamma. and IFN.alpha.. MCF, purified by dye ligand and ion-exchange chromatography, comprises two polypeptides of 29 and 14.7kD (P29 and P14.7) which can be further purified by preparative SDS/PAGE and hydrophobic chromatography, respectively. Amino acid composition analyses and immunoblotting of two-dimensional gels indicate that these are distinct but possibly related polypeptides. N-terminal analysis of P29 reveals it to be a previously undescribed cytokine mediator of monocyte function. A peptide having the sequence Gly Ala Ala Val Leu Glu Asp Ser Gln, corresponding to the native N-terminal sequence of P29, also exhibits MCF activity equivalent to that of the intact 29kD protein.
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Board of Regents , The University of Texas System
Hill Jr. Robert J.
Salata Carol A.
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