Synthetic resins or natural rubbers -- part of the class 520 ser – Synthetic resins – Mixing of two or more solid polymers; mixing of solid...
Patent
1992-05-12
1993-11-02
Nutter, Nathan M.
Synthetic resins or natural rubbers -- part of the class 520 ser
Synthetic resins
Mixing of two or more solid polymers; mixing of solid...
530333, 530334, C08F28300, C08G 6348, C08G 6391
Patent
active
052584542
ABSTRACT:
A method for the solid-phase synthesis of peptides or proteins in high yield and high purity uses a solid-support consisting of a functionalized polystyrene-grafted polymer substrate, the grafted polystyrene chains being substantially non-cross-linked and having a chain molecular weight, not including optional non-reactive substituents, of at least 200,000, preferably in the range of 600,000-1,200,000. Particularly suitable polymer substrates are substrates of a polyolefin such as polyethylene. The method is particularly well-suited to the compartmentalized synthesis of a multitude of peptides or proteins in a parallel and substantially simultaneous fashion.
Preferred embodiments of a solid support for performing the synthesis are prepared from thin polyethylene sheet or film which has been grafted with polystyrene chains in a radical-initiated process in which the polyethylene sheet or film is immersed in a solution of optionally substituted styrene monomer in an alcohol such as methanol, the volume percentage of styrene in the solution preferably being about 30% v/v, and subjected to gamma irradiation.
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Effect of Swelling on Radiation-Induced Grafting of Styrene to Polyethylene, Sueo Machi, Ihab Kamel and Joseph Silverman, (1970).
Garnett and Yen American Counsel Society 1980 "Acid Effects In The Radiation Grafting Of Monomers To Polymers, Particularly Polyethylene".
Odian et al Journal of Applied Polymer Science vol. 7, pp. 245-250 (1963) "Accelerative Effects In Radiation-Induced Graft Polymerization".
Odian et al Journal of Polymer Science, vol. XLII Issue 140 (1960) "The Accelerating Effect of Methanol on the Gamma Radiation Induced Graft Copolymerization of Styrene to Polyethylene".
Odian et al Journal of Polymer Science vol. 55, pp. 663-673 (1961) "Radiation-Induced Graft Polymerization: The Trommsdorff Effect of Methanol".
Almdal Kristoffer
Berg Rolf H.
Holm Arne
Merrifield Robert B.
Pedersen Walther B.
Nutter Nathan M.
Riso National Laboratory
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