Peptide synthesis and purification by fusion to penI protein or

Details Peptide synthesis and purification by fusion to penI protein or Peptide synthesis and purification by fusion to penI protein or

1994-04-29

1999-03-30

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 72, 4352523, 43525233, 4353707, 4241851, 536 234, 530350, A61K 3900, C12N 1562, C07K 100, C07K 1900

Patent

active

058887750

ABSTRACT:
The present invention provides novel methods for the synthesis and isolation and purification of a peptide of interest (target peptide). In particular, the invention relates to peptide synthesis, isolation and purification methods that comprise use of penI fusion polypeptides and related gene fusion constructs that encode such polypeptides.

REFERENCES:
patent: 4952508 (1990-08-01), Chang et al.
patent: 5215896 (1993-06-01), Keck et al.
patent: 5258502 (1993-11-01), Kuranda
Smith, et al., Gene, 67:31-40; Method and Vector Organism for Controlled Accumulation of the Cloned Heterologous Gene Production Bacillus Subtilis (1988).
P. Schatz, "Use of Peptide Libraries to Map the Substrate Specificity of a Peptide-Modifying Enzyme: A 13 Residue Consensus Peptide Specifies Biotinylation in Escherichia coli", Bio/Technology, vol. 11, pp. 1138-1143 (Oct. 1993).
D. J. Sherratt, et al., "Analysis by Transformation of the Penicillinase System in Bacillus licheniformis", Journal of General Microbiology, vol. 76, pp. 217-230 (1973).
D. A. Dubnau, et al., "The Genetics of Bacillus licheniformis Penicillinase: a Preliminary Analysis from Studies on Mutation and Inter-strain and Intra-strain Transformation", J. gen. Microbiol., vol. 41, pp. 7-21 (1965).
T. Himeno, et al., "Nucleotide Sequence of the Penicillinase Repressor Gene penI of Bacillus licheniformis and Regulation of penP and penI by by the Repressor", Journal of Bacteriology, vol. 168, No. 3, pp. 1128-1132 (Dec. 1986).
V. Wittman, et al., "Regulation of the Penicillinase Genes of Bacillus licheniformis: Interation of the pen Repressor with Its Operators", Journal of Bacteriology, vol. 170, No. 7, pp. 3206-3212 (Jul. 1988).
Imanaka, T. et al., 1981, Journal of Bacteriology, 147(3):776-788.
Imanaka, T., et al., 1992, Journal of Bacteriology, 174(4):1423-1425.
Imanaka, T., et al., 1993, Journal of Fermentation and Bioengineering 76(1):1-6.
Wong, A.C., et al., 1990, in Genetics and Biotechnology of Bacilli, vol. 3, Zukowski, et al., Eds., pp. 115-122.
Hiramatsu, K., et al., 1992, FEBS Letters, 298(2-3):133-136.
Wittkman, V., et al., 1993, Journal of Bacteriology, 175(22):7383-7390.

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