Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1985-06-13
1990-05-08
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435810, 530326, 530327, 530328, 530329, 530330, C12Q 148
Patent
active
049238027
ABSTRACT:
A highly specific peptide substrate for protein kinase C is composed in basic form of a serine or threonine amino acid residue flanked by groups of basic amino acids composed entirely of argenine, lysine or histidine or any combination of these amino acid residues. The peptide, which is phosphorylated by the protein kinase C, can be used in a direct or competitive assay for protein kinase C by employing radiolabeled ATP. In the phosphorylation process the phosphate is transferred from the ATP by the protein kinase C to the peptide of the present invention.
REFERENCES:
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Shackelford, D. A. and I. A. Trowbridge, "Induction of Expression and Phosphorylation of the Human Interleukin 2 Receptor by a Phorbol Diester," J. Biol. Chem., 259:11706 (1984).
Wise, Bradley C. et al., "Phospholipid-Sensitive Ca2+-Dependent Protein Kinase from Heart, II, Substrate Specificity and Inhibition by Various Agents," J. Biol. Chem., 257:8489 (1982).
Leonard, Warren J., "Molecular Cloning and Expression of cDNAs for the Human Interleukin-2 Receptor," Nature 311:626 (1984).
Ramachandran, Chidambaram et al., "Phosphorylation of High Mobility-Group Proteins by the Calcium-Phospholipid-Dependent Protein Kinase . . . " J. Biol. Chem. 259:13495 (1984).
Hunter, et al., "Protein Kinase C Phosphorylation of the EGF Receptor at a Threonine Residue Close to the Cytoplasmic Face of the Plasma Membrane", Nature 311:480 (1984).
Niedel, et al., "Phorbol Diester Receptor Copurifies with Protein Kinase C", Pro. Natl. Acad. Sci. U.S.A. 80:36 (1983).
Castagna, et al., "Direct Activation of Calcium-Activated, Phospholipid-Dependent Protein Kinase by Tumor-Promoting Phorbol Esters", J. Bio. Chem. 257:7847 (1982).
Leach, et al., "Characterization of a Specific Phorbol Ester Aporeceptor in Mouse Brain Cytosol", Proc. Natl. Acad. Sci. U.S.A. 80:4208 (1983).
Parker, et al., "Purification to Homogeneity of Protein Kinase C from Bovine Brain-Identity with the Phorbol Ester Receptor", EMBO Journal 3:953 (1984).
Immunex Corporation
Scheiner Toni R.
Warden Robert J.
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