Peptide purification process

Details Peptide purification process Peptide purification process

1991-11-12

1993-10-05

Cashion, Jr., Merrell C.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Separation or purification

530331, A61K 3702, C07K 500

Patent

active

052506606

ABSTRACT:
Tripeptides, D-Phe-L-Pro-L-Arg-H, D-Phg-L-Pro-L-Arg-H and related compounds are purified in a process comprising 1) HPLC chromatography over an alkylsilane resin and elution with a gradient comprising an organic phase of between about 2% and about 40% of acetonitrile in an aqueous acidic phase containing an inorganic acid e.g. H.sub.2 SO.sub.4 and HCl, at a pH between about 2 and about 3, and 2) adjusting the pH of the acidic eluate with a water insoluble basic ion-exchange resin to about 4 to about 6.5. The purified peptides are useful antithrombotic agents.

REFERENCES:
patent: 4377482 (1983-03-01), Rivier
patent: 4399065 (1983-08-01), Bajusz et al.
patent: 4533494 (1985-08-01), Uchiyama et al.
Tomeri et al. Chromatographia, vol. 19 (1984) 437-442.

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