Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Separation or purification
Patent
1995-01-27
1997-07-15
Lukton, David
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
Separation or purification
530334, 530337, 530345, 530336, A61K 3804
Patent
active
056484623
ABSTRACT:
Disclosed is a method of purifying a mature peptide with a free N-terminus group from a mixture of peptides comprising the mature peptide and immature end-capped peptides. The peptides are bound to a solid-phase support and are produced by solid phase synthesis. The mixture is contacted with a linker comprising a functional group at each terminus. The functional group at one terminus has the structure --SO.sub.2 --CH.sub.2 --CH.sub.2 --X, wherein X is an activated carbonate group which reacts selectively with N-terminus of the mature peptide to form a urethane linkage. The functional group at the other terminus is a thiol protected with an acid labile group. After cleaving the peptides from the solid support and thiol protecting group with acid, the mature peptide is separated from the mixture by forming a covalent bond between the liberated thiol and a solid support. Treatment with base cleaves the mature peptide from the linker to give a purified mature peptide.
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Krieger Proc Natl Acad Sci 73 3160 1976.
Krieger, D.E., et al., "Affinity Purification of Synthetic Peptides," Proc. Natl. Acad. Sci. USA, 73:3160-3164 (1976).
Fujii, N., et al., "Studies on Peptides. CLV. Evaluation of Trimethylsilyl Bromide as a Hard-Acid Deprotecting Reagent in Peptide Synthesis," Chem. Pharm. Bull., 35:3880-3883 (1987).
Funakoshi, S., et al., "Chemoselective One-Step Purification Method for Peptides Synthesized by the Solid-Phase Technique," Proc. Natl. Acad. Sci., 88:6981-6985 (1991).
Sucholeiki, I., and Lansbury, P.T., Jr., "Affinity Chromatographic Method for the Purification of Water-Insoluble Peptides," J. Org. Chem., 5478-5484.
Fukuda Hiroyuki
Funakoshi Susumu
Lukton David
Setsuko Funakoshi
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