Peptide purification

Details Peptide purification Peptide purification

2006-06-06

2006-06-06

Campell, Bruce R. (Department: 1654)

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

8 to 10 amino acid residues in defined sequence

C514S002600

Reexamination Certificate

active

07057014

ABSTRACT:
A nona- or decapeptide is purified from residual organic solvent by dissolving in a solvent comprising water and at least one C1–C3alcohol followed by precipitation into a vigorously stirred solvent consisting of an alkyl ester of a carboxylic acid, the ester comprising from 3 to 6 carbon atoms, and one or several non-polar compounds selected from hexane, heptane, octane, cyclohexane, methylcyclohexane, and, optionally, of up to 5% of acetic or propionic acid, isolating the precipitated nona- or decapeptide, followed by washing with a mixture of C3–C5esters and drying, with the provisio that the water content of the solvent comprising water and the at least one alcohol is below 8% (v/v), and that the volume ratio of the dissolution solvent mixture and the precipitation solvent mixture is 1:10 or higher. Also described is the monoacetate of Ac-D-2Nal-D-4ClPhe-D-3Pal-Ser-MeTyr-D-Asn-Leu-Lys(iPr)-Pro-D-Ala-NH2.

REFERENCES:
patent: 0 136 728 (1985-04-01), None
patent: WO-00/18423 (2000-04-01), None
patent: WO 00/18423 (2000-06-01), None
Sakamoto, et al., Journal of Antibiotics, 1993, 46, 1788-1798.

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