Peptide plasminogen activators

Details Peptide plasminogen activators Peptide plasminogen activators

1995-01-31

1997-08-12

Jacobson, Dian C.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

435212, 435226, 4353201, 536 232, A61K 3849, C12N 964, C12N 1555, C12N 1563

Patent

active

056562691

ABSTRACT:
Novel polypeptide compositions based on the amino acid sequence of tissue plasminogen activator (tPA) are provided having improved properties over natural tissue plasminogen activator. Particularly, reduced fibrin stimulation of plasminogenolytic activity and/or enhanced affinity to fibrin surfaces are provided by modifying the C-terminus by deletions.

REFERENCES:
patent: 4751084 (1988-06-01), Feder et al.
patent: 4753879 (1988-06-01), Rosa et al.
patent: 5073494 (1991-12-01), Heyneker et al.
patent: 5106741 (1992-04-01), Marotti et al.
patent: 5147643 (1992-09-01), Heyneker et al.
patent: 5258180 (1993-11-01), Gill
patent: 5344773 (1994-09-01), Wei et al.
Haigwood, Nancy L. et al., "Variants of Human Tissue-Type Plasminogen Activator Substituted at the Protein Cleavage Site and Glycosylation Sites, and Truncated at the N-and C-termini," Protein Engineering, vol. 2, No. 8, pp. 611-620 (1989).
Craik, Charles S. et al., "Redesigning Trypsin: Altera-of Substrate Specificity," Science, vol. 228, pp. 291-297 (Apr. 1985).
Rosenberg, Steven et al., "Synthesis in yeast of a functional oxidation-resistant mutant of human .alpha..sub.1 -antitrypsin," Nature, vol. 312, No. 5989, pp. 77-80 (Nov. 1984).
Wilkinson, Anthony J. et al., "A large increase in enzyme-substrate affinity by protein engineering," Nature, vol. 307, pp. 187-188 (Jan. 1984).
Andreasen, P.A., "Inactive proenzyme to tissue-type plasminogen activator from human melanoma cells, identified after affinity purification with a monoclonal antibody," EMBO Journal, vol. 3, pp. 51-55 (1984).
Nye et al., Figure I, Proc. Natl. Acad. Sci. USA, vol. 81, p. 5355 (1984).
van Zonneveld, A.J., Abstract from ISTH Meeting, Jul. 14-19, 1985, report on the relationship between structure and function of human tissue-type plasminogen activator.
Opdenakker, G., et al., "The Carbohydrate Side-Chains of Tissue-Type Plasminogen Activator Influences its Activity," EMBO Workshop on Plasminogen Activation, Emalfi, Italy, Oct. 14-18, 1985.
Loskutoff, D.J. et al., "Detection of an unusually stable fibrinolytic inhibitor produced by bovine endothelial cells," Proc. Natl. Acad. Sci. USA, vol. 80, pp. 2956-2960 (May 1963).
Loskutoff, D.J., "Thrombosis and Haemostasis," Journal of the International Society of Thrombosis and Haemostasis, vol. 54, No. 1, p. 118 (S699) (1986).
Zoller, Mark J. et al., "Oligonucleotide-Directed Mutagenesis of DNA Fragments Cloned into M13 Vectors," Methods in Enzymology, vol. 100, pp. 468-500 (1983).
Pohl, Gunnar et al., "Tissue Plasminogen Activator: Peptide Analyses Confirm an Indirectly Derived Amino Acid Sequence, Identify the Active Site Serine Residue, Establish Glycosylation Sites, and Localize Variant Differences," Biochemistry, vol. 23, pp. 3701-3707 (1984).
Zoller, Mark J. et al., "Oligonucleotide-directed mutagenesis using M13-derived vectors; an efficient and general procedure for the production of point mutations in any fragment of DNA," Nucleic Acids Research, vol. 10, No. 20 (Aug. 1982).
Guan, Jun-Lin et al., "Glycosylation Allows Cells-Surface Transport of an Anchored Secretory Protein," Cell, vol. 42, pp. 489-496 (Sep. 1965).
Little, Sheila P. et al., "Functional Properties of Carbohydrate-Depleted Tissue Plasminogen Activator," Biochemistry, vol. 23, pp. 6191-6195 (1984).
Nose, Masato et al., "Biological significance of carbohydrate chains on monoclonal antibodies," Proc. Natl. Acad. Sci. USA, vol. 80, pp. 6632-6636 (Nov. 1983).
Stryer, L., Chapter 8, Zymogen Activation, Biochemistry pp. 165-166 (1988).
NY, Tor et al., "The structure of the human tissue-type plasminogen activator gene: Correlation of intron and exon structures so functional and structural domains," Proc. Natl. Acad. Sci., vol. 81, pp. 5355-5359 (1984).
Vehar, Gordon A. et al., "Characterization Studies on Human Melanoma Cell Tissue Plasminogen Activator," Biotechnology, vol. 2, pp. 1051-1057 (1986).
Pennica, Diane et al., "Cloning and Expression of Human Tissue-Type Plasminogen Activator cDNA in E.coli," Nature, vol. 301, No. 5897, pp. 214-221 (1983).

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