Chemistry of carbon compounds – Miscellaneous organic carbon compounds
Patent
1976-05-07
1977-12-27
Phillips, Delbert R.
Chemistry of carbon compounds
Miscellaneous organic carbon compounds
23253R, 2601125R, C08L 3700
Patent
active
040654125
ABSTRACT:
A method and apparatus for the sequential degradation of protein or peptide molecules by successive coupling and cleavage reactions. Such molecules are immobilized on a macroporous reaction support surface and placed in a flowthrough reaction chamber which is mounted in a sequencer. In the Edman sequencing technique, reagents driven by pressurized inert gas are passed through the reaction chamber as follows: (a) liquid or vapor coupling reagent, (b) coupling base vapor, (c) washing solvent, (d) inert gas to partially dry the support surface, (e) cleavage reagent vapor to cleave amino acid derivatives from the immobilized coupled protein or peptide chains, (f) liquid extracting solvent to withdraw the cleaved amino acid derivative. An automated sequencer to carry out said method.
REFERENCES:
patent: 3725010 (1973-04-01), Penhast
Edman, P., Acta Chem. Scand. 4, 283-293, 1950.
Biochem. et Biophys. Acta 115, 371-396, 1966.
Methods in Enzymology 11, 455, 461-469, 1967.
Proc. First Int'l. Conf. on Meth. in Protein Sequence Anal., 193 (1975).
Eur. J. Biochem. 1, 80 (1967).
Physiol. Chem. 354, 1415 (1973).
Eur. J. Biochem. 20, 89 (1971).
Febs. Lett. 35, 97 (1973).
Durrum Instrument Corporation
Phillips Delbert R.
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