Peptide-mediated transfection agents and methods of use

Details Peptide-mediated transfection agents and methods of use Peptide-mediated transfection agents and methods of use

2005-01-11

2005-01-11

Wax, Robert A. (Department: 1653)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

C514S012200, C530S324000, C530S326000

Reexamination Certificate

active

06841535

ABSTRACT:
Peptides that are useful as transfection agents are described. The peptides can facilitate the efficient cellular internalization of a broad range and size of compounds that when non-covalently complexed therewith are efficiently internalized into a cell. Advantages include but are not limited to excellent transfection efficiency, relatively low toxicity, internalization by a broad host cell spectrum, and the simplicity and cost-effectiveness that arise from not having to covalently complex the peptide with a specific molecule to be delivered. Applications include but are not limited to the delivery of diagnostics and therapeutics, as well as drug discovery, gene discovery, and the analysis and/or manipulation of other cellular and molecular biological functions. Claims are made for transfection agents, compositions of matter, including pharmaceutical compositions, reagent kits, methods of delivery, and methods of identification of additional peptides for performing and/or including in the same.

REFERENCES:
patent: 5270163 (1993-12-01), Gold et al.
patent: 5747253 (1998-05-01), Ecker et al.
patent: WO 0018778 (2000-04-01), None
Arar et al. (1995), “Synthesis and Antiviral Activity of Peptide-Oligonucleotide Conjugates Prepared by Using N-(Bromoacetyl) Peptides,” Bioconjug. Chem., 6, 573-5772.
Beven et al. (1997), “Effects on Mollicutes (Wall-less Bacteria) of Synthetic Peptides Comprising a Signal Peptide or a Membrane Fusion Peptide, and a Nuclear Localization Sequence (NLS)—A Comparison with Melittin,” Biochim. Biophys. Acta, 1329, 357-369.
Bongartz et al. (1994), “Improved Biological Activity of Antisense Oligonucleotides Conjugated to a Fusogenic Peptide,” Nucleic Acids Res., 22, 4681-4688.
Briggs and Gierasch (1986), “Molecular Mechanisms of Protein Secretion: The Role of the Signal Sequence,” Adv. Prot. Chem. 38, 109-180.
Brugidou et al. (1995), “The Retro-Inverso Form of a Homeobox-Derived Short Peptide is Rapidly Internalised by Cultured Neurones: A New Basis for an Efficient Intracellular Delivery System,” Biochem. Biophys. Res. Commun., 214, 685-693.
Chaloin et al. (1998), “Design of Carrier Peptide-Oligonucleotide Conjugates with Rapid Membrane Translocation and Nuclear Localization Properties,” Biochem. Biophys. Res. Commun., 243, 601-608.
Chaloin et al. (1997), “Synthetic Peptides as Carriers for Cellular Import of Drugs,” Lett. Pept. Sci., 4, 231-234.
Chaloin et al. (1997), “Conformations of Primary Amphipathic Carrier Peptides in Membrane Mimicking Environments,” Biochemistry, 36, 11179-11187.
Chaloin et al. (1998), “Ionic Channels Formed by a Primary Amphipathic Peptide Containing a Signal Peptide and a Nuclear Localization Sequence,” Biochim Biophys. Acta, 1375, 52-60.
Chen et al. (1999), “Selective Killing of Transformed Cells by Cyclin/Cyclin-Dependent Kinase 2 Antagonists,” Proc. Natl. Acad. Sci. USA, 96:4325-4329.
Degols et al. (1989), “Antiviral Activity and Possible Mechanisms of Action of Oligonucleotides-poly (L-lysine) Conjugates Targeted to Vesicular Stomatitis Virus mRNA and Genomic RNA,” Nucleic Acids Res., 17: 9341-9350.
Derossi et al., (1996), “Cell Internalization of the Third Helix of the Antennapedia Homeodomain is Receptor-Independent,” J. Biol. Chem., 271, 18188-18193.
Dingwall, C. and Laskey, R. (1992), “The Nuclear Membrane,” Science, 258, 942-947.
Felgner et al., (1987), “Lipofection: A Highly Efficient, Lipid-Mediated DNA-Transfection Procedure,” Proc. Natl. Acad. Sci. USA, 84, 7413-7417.
Freed et al. (1990), “Characterization of the Fusion Domain of the Human Immunodeficiency Virus Type 1 Envelope Glycoprotein gp41,” Proc. Natl. Acad. Sci. USA, 87, 4650-4654.
Gallaher, W.R. (1987), “Detection of a Fusion Peptide Sequence in the Transmembrane Protein of Human Immunodeficiency Virus,” Cell, 50, 327-328.
Goldfarb et al., (1986), “Synthetic Peptides as Nuclear Localization Signals,” Nature (London), 322, 641-644.
Gottschalk et al. (1996), “A Novel DNA-peptide Complex for Efficient Gene Transfer and Expression in Mammalian Cells,” Gene Ther., 3, 448-457.
Haensler and Szoka (1993), “Polyamidoamine Cascade Polymers Mediate Efficient Transfection of Cells in Culture,” Bioconjugate Chem., 4, 372-379.
Harris, J.D. and Lemoine, N.R. (1996), “Strategies for Targeted Gene Therapy,” Trends Genet., 12, 400-405.
Kalderon et al., (1984), “Sequence Requirements for Nuclear Location of Simian Virus 40 Large-T Antigen,” Nature (London), 311, 33-38.
Lewis et al. (1996), “A Serum-Resistant Cytofectin for Cellular Delivery of Antisense Oligodeoxynucleotides and Plasmid DNA,” Proc. Natl Acad. Sci. USA, 93, 3176-3181.
Méry et al. (1993), “Disulfide Linkage to Polyacrylic Resin for Automated Fmoc Peptide Synthesis,” Int. J. Peptide Prot. Res., 42, 44-52.
Morris et al. (1997), “A New Peptide Vector for Efficient Delivery of Oligonucleotides into Mammalian Cells,” Nucleic Acids Res., 25, 2730-2736.
Morris et al. (1999), “A Novel Potent Strategy for Gene Delivery Using a Single Peptide Vector as a Carrier,” Nucleic Acids Res., 27, 3510-3517.
Morris et al. (1999), “A New Potent HIV-1 Reverse Transcriptase Inhibitor,” J. Biol. Chem., 274, 24941-24946.
Morris et al. (2000), “Translocating Peptides and Proteins and Their Use for Gene Delivery,” Curr. Opinion in Biotech., 11, 461-466.
Morris et al. (2000), “An Essential Phosphorylation-site Domain of Human cdc25C Interacts with Both 14-3-3 and Cyclins,” J. Biol. Chem., 275:28849-28857.
Morris et al. (2001), “A Peptide Carrier for the Delivery of Biologically Active Proteins Into Mammalian Cells,” Nature Biotech. 19, 1173-1176.
Niidome et al. (1997), “Binding of Cationic a-Helical Peptides to Plasmid DNA and Their Gene Transfer Abilities Into Cells,” J. Biol. Cham., 272, 15307-15312.
Pasqualini and Ruoshlahtl (1996), “Organ Targeting In Vivo Using Phage Display Peptide Libraries,” Nature 380:364-366 (1999).
Phelan et al. (1998), “Intercellular Delivery of Functional p53 by the Herpesvirus Protein VP22,” Nat. Biotechnol., 16:440-443.
Prabhakaran, “The Distribution of Physical, Chemical, and Conformational Properties in Signal and Nascent Peptides,” Biochem. J. (1990) 269:691-696.
Pichon et al. (1997), “Intracellular Routing and Inhibitory Activity of Oligonucleopeptides Containing a KDEL Motif,” Mol. Pharmacol., 51, 431-438.
Plank et al. (1994), “The Influence of Endosome-Disruptive Peptides on Gene Transfer Using Synthetic Virus-like Gene Transfer Systems,” J. Biol. Chem., 269, 12918-12924.
Sheldon et al. (1995), “Loligomers: Design of de novo Peptide-based Intracellular Vehicles,” Proc. Natl. Acad. Sci. USA, 92, 2056-2060.
Van Mau et al. (1999), “Lipid-Induced Organization of a Primary Amphipathic Peptide: A Coupled AFM-Monolayer Study,” J. Membrane Biol., 167, 241-249.
Vidal et al. (1997), “Efficient RNA Delivery into Non-transformed Mammalian Cells by Using a Peptide Vector,” Comptes Rendus Acad. Sci. Paris, 320, 279-287.
Vidal et al. (1997), “Conformations of a Synthetic Peptide Which Facilitates the Cellular Delivery of Nucleic Acids,” Lett. Peptide Sci., 4, 227-230.
Vidal et al. (1998), “Interactions of Primary Amphipathic Vector Peptides with Membranes—Conformational Consequences and Influence on Cellular Localization,” J. Membrane Biol., 162, 259-264.
Vives et al. (1997), “A Truncated HIV-1 Tat Protein Basic Domain Rapidly Translocates through the Plasma Membrane and Accumulates in the Cell Nucleus,” J. Biol. Chem., 272, 16010-16017.
Wagner et al. (1992), “Influenza Virus Hemagglutinin HA-2 N-Terminal Fusogenic Peptides Aug

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