Peptide-mediated gene transfer

Details Peptide-mediated gene transfer Peptide-mediated gene transfer

1994-05-11

1997-09-23

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4351723, 4352402, 4353201, 935 55, C12N 1500, C12N 506, C12N 1563

Patent

active

056703473

ABSTRACT:
A methodology that allows for highly efficient transfer and stable integration of DNA into both established eukaryotic cell lines and primary cells, including non-dividing cells such as human peripheral blood monocytes and macrophages, entails the use of a synthetic polypeptide comprised of a peptide domain which corresponds to a nuclear localization signal sequence and a DNA binding domain which is rich in basic amino acids, separated by a hinge region of neutral amino acid which prevents stearic interference between the two domains.

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