Peptide ligands for affinity purification of fibrinogen

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 6 to 7 amino acid residues in defined sequence

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530382, A61K 3808, C07K 706

Patent

active

057836639

ABSTRACT:
Peptides which bind to fibrinogen are disclosed. These peptides have available fibrinogen binding domains having a Trp-Gln-Glu-His-Tyr-Asn, Trp-Gln-Glu-Thr-TyrGln,or Tyr-Glu-Asn-Tyr-Gly-Tyr sequence. Peptides containing at least one of these fibrinogen binding domains are immobilized upon a chromatographic substrate in a preferred embodiment of the invention. This preferred embodiment is useful in an affinity chromatography process to purify fibrinogen.

REFERENCES:
Baumbach et al. Protein purification using affinity ligands from peptide libraries. BioPharm. 24-35, May 1992.
Buettner et al. Chemically derived peptide libraries: A new resin and methodology for lead identification. Int. J. Peptide Protein Res. 47:70-83, 1996.
Smith et al. A ribonuclease S-peptide antagonist discovered with a bacteriophage display library. Gene 128:37-42, 1993.
Brennan, M. Fibrin glue. Blood Reviews 5:240-244, 1991.

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