Peptide fragmentation

Details Peptide fragmentation Peptide fragmentation

2006-07-11

2006-07-11

Le, Long V. (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S069700, C436S119000, C558S044000

Reexamination Certificate

active

07074570

ABSTRACT:
The present invention relates to a method of identifying a polypeptide using a class of novel, water-stable reagents. More specifically, the method according to the invention comprises the steps of(a) derivatization in an aqueous solution of the N-terminus of the polypeptide, or the N-termini of one or more peptides of the polypeptide, with at least one acidic reagent which comprises a sulfonyl moiety coupled to an activated acid moiety to provide one or more peptide derivatives;(b) analyzing at least one such derivative using a mass spectrometric technique to provide a fragmentation pattern; and(c) interpreting the fragmentation pattern.Furthermore, the present invention also relates to a kit for identifying a polypeptide by a mass spectrometric technique, which kit comprises at least one acidic reagent comprising a sulfonyl moiety coupled to an activated acid moiety in a container together with suitable means for analysis of the fragmentation pattern obtained.

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