Chemistry: analytical and immunological testing – Nuclear magnetic resonance – electron spin resonance or other...
Reexamination Certificate
2006-07-27
2010-11-23
Gakh, Yelena G (Department: 1797)
Chemistry: analytical and immunological testing
Nuclear magnetic resonance, electron spin resonance or other...
C436S086000, C436S089000
Reexamination Certificate
active
07838303
ABSTRACT:
A method of facilitating analysis of a peptide in a mass spectrometer comprising derivatizing the C-terminus of the peptide with an amino acid residue via a reaction with a carbodiimide reagent, yielding a derivative peptide, ionizing the derivative peptide with a double charge, and fragmenting the ionized derivative peptide in a mass spectrometry system, wherein binary fragments of the ionized derivative each include a charge, facilitating sequence analysis of the peptide.
REFERENCES:
patent: 2003/0119069 (2003-06-01), Schneider
patent: 2003/0153007 (2003-08-01), Chen et al.
patent: 2004/0161795 (2004-08-01), Marto
patent: 2004/0209251 (2004-10-01), Schneider
Carter “Vonjugate of Peptodes to Carrier Protein via Carbodiimide” from “The Protein Protocols Handbook”, 1996, p. 693.
GB Search Report, Nov. 6, 2007.
T. Nakazawa et al., “Enhanced responses in matrix-assisted laser desorption/ionization mass spectrometry of peptides derivatized with arginine via a C-terminal oxazolone”, Rapid Communications in Mass Spectrometry, vol. 18, pp. 799-807 (2004).
Fischer Steven M.
Perkins Patrick D.
Agilent Technologie,s Inc.
Gakh Yelena G
LandOfFree
Peptide derivatization method to increase fragmentation... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Peptide derivatization method to increase fragmentation..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Peptide derivatization method to increase fragmentation... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4196753