Peptide derivatives and use thereof as substrates for quantitati

Details Peptide derivatives and use thereof as substrates for quantitati Peptide derivatives and use thereof as substrates for quantitati

1984-06-01

1986-12-16

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

2609982, 435 4, 530331, 530802, C12Q 144

Patent

active

046296956

ABSTRACT:
Peptide derivatives of formula ##STR1## wherein R.sup.1 represents a substituted amino group which is capable of being split off enzymatically with formation of a colored or fluorescent compound R.sup.1 -H. The said peptide derivatives are used for quantitatively assaying the enzyme C.sub.1 -esterase. The assaying is carried out by reacting a medium which contains C.sub.1 -esterase or in which the latter is formed or consumed with a peptide derivative as defined above and measuring photometrically, spectrophotometrically, fluorescence-spectrophotometrically or electrochemically the quantity of split product R.sup.1 -H released per time unit by the catalytic hydrolytic action of the said enzyme on the peptide derivative.

REFERENCES:
patent: 4217269 (1980-08-01), Cole
patent: 4221706 (1980-09-01), Ali et al.
patent: 4279810 (1981-07-01), Claeson et al.
patent: 4379764 (1983-04-01), Fujii et al.

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