Peptide conjugates for the stabilization of membrane...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...

Reexamination Certificate

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Reexamination Certificate

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07087729

ABSTRACT:
The present invention provides a novel class of detergents referred to herein as lipopeptide detergents. Lipopeptide detergents comprise an amphipathic α-helical peptide having a hydrophobic or neutral face and a hydrophilic face. To each end of this peptide is covalently linked an aliphatic hydrocarbon tail, these aliphatic tails being linked thereto such that they associate with the hydrophobic or neutral face of the peptide. Lipopeptide detergents can advantageously be used to stabilize membrane proteins in the absence of a phospholipid bilayer in a manner that preserves the native conformation and permits the subsequent crystallization thereof.

REFERENCES:
patent: 99/55383 (1999-11-01), None
Online-Medical Dictionary. “Amino acid”. http://cancerweb.ncl.ac.uk/cgi-bin/omd?query=amino+acid. Nov. 13, 1997.
P. Berndt, et al., “Synthetic lipidation of peptides and amino acids: monolayer structure and properties” Journal of the American Chemical Society, vol. 117, No. 37, 1995, pp. 9515-9522.
Christian E. Schafmeister, et al., “Structure at 2.5 A of a Designed Peptide that Maintains Solubility of Membrane Proteins” Science, vol. 262, Oct. 29, 1993.

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