Peptide conjugates and fluorescence detection methods for...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

Reexamination Certificate

active

06979530

ABSTRACT:
Polypeptides labelled with a donor and acceptor pair of dyes selected from a dibenzorhodamine dye and a diamino-benzophenoxazine dye are peptide conjugates which are useful for intracellular and bead-based assays with fluorescence detection. Peptide conjugates with a caspase-recognition site undergo cleavage into peptide fragments which may be detected, located, and quantitated by the changes in fluorescence. Intracellular cleavage of peptide conjugates is correlated with apoptosis.

REFERENCES:
patent: 5547849 (1996-08-01), Baer et al.
patent: 5605809 (1997-02-01), Komoriya et al.
patent: 5698411 (1997-12-01), Lucas et al.
patent: 5714342 (1998-02-01), Komoriya et al.
patent: 5936087 (1999-08-01), Benson et al.
patent: 5976822 (1999-11-01), Landrum et al.
patent: 5989835 (1999-11-01), Dunlay et al.
patent: 6051719 (2000-04-01), Benson et al.
patent: 6103479 (2000-08-01), Taylor
patent: 6127139 (2000-10-01), Te Koppele et al.
patent: 6130745 (2000-10-01), Manian et al.
patent: 6166202 (2000-12-01), Simmonds et al.
patent: 6181413 (2001-01-01), Manian
patent: 6235493 (2001-05-01), Bissell et al.
patent: 6248904 (2001-06-01), Zhang et al.
patent: 6583168 (2003-06-01), Menchen et al.
patent: 1 033 411 (2000-09-01), None
patent: WO 97/29154 (1997-08-01), None
patent: WO 99/18856 (1999-01-01), None
patent: WO 00/50635 (2000-08-01), None
patent: WO 00/64988 (2000-11-01), None
patent: WO 00/73437 (2000-12-01), None
patent: WO 00/75160 (2000-12-01), None
patent: WO 02/044416 (2002-06-01), None
International Search Report from PCT/US02/15713 mailed Dec. 18, 2003.
“CaspaTag™ Fluorescence Labeled Caspase Activity Kit,” Product Profile, Intergen Company.
“Apoptag® In Situ Apoptosis Detection Kits,” Product Profile, Intergen Company.
“Apoptag™ Red In Situ Apoptosis Detection Kit,” Product Profile, Intergen Company.
Miraglia et al., “Homogeneous Cell-and Bead-Based Assays for High Throughput Screening Using Fluorometric Microvolume Assay Technology,”Journal of Biomolecular Screening, vol. 4, No. 4, pp. 193-204, 1999.
Swartzman et al., “A Homogeneous and Multiplexed Immunoassay for High-Throughput Screening Using Fluorometric Microvolume Assay Technology,”Analytical Biochemistry, 271:143-151 (1999).
Thornberry et al., “Caspases: Enemies Within,”Science, vol. 281, pp. 1312-1316, Aug. 28, 1998.
Thornberry et al., “A Combinatorial Approach Defines Specificities of Members of the Caspase Family and Granzyme B,”The Journal of Biological Chemistry, vol. 272, No. 29, pp. 17907-17911, 1997.
Xu et al., “Detection of programmed cell death using fluorescence energy transfer,”Nucleic Acids Research, vol. 26, No. 8, pp. 2034-2035, 1998.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Peptide conjugates and fluorescence detection methods for... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Peptide conjugates and fluorescence detection methods for..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Peptide conjugates and fluorescence detection methods for... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3505939

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.