Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Reexamination Certificate
2004-05-26
2009-08-04
Mondesi, Robert B (Department: 1645)
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
C530S300000, C530S333000
Reexamination Certificate
active
07569537
ABSTRACT:
A process to identify peptide antagonists of Hsp16.3, a chaperon protein necessary for the survival ofMycobacterium tuberculosisin the dormant phase is described. Affinity selection of a 7-mer and a 12-mer random peptide libraries displayed on bacteriophage M13 was performed using recombinant Hsp16.3 as template and two peptide phage clones, which bind to the Hsp16.3 protein were identified. Synthetic peptides corresponding to the peptide sequences displayed on these phage clones were able to specifically bind and inhibit the chaperone function of Hsp16.3 in vitro in a dose dependent manner. The corresponding inhibitory effect of these peptides on the chaperon activity of alphaB-crystallin, a constituent of human eye lens and a homologue of Hsp16.3, was found to be substantially less. These peptide inhibitors, or similar inhibitors generated by the process described, which specifically target Hsp16.3, can hence be used as lead compounds to obtain better therapeutics against latenttuberculosis.
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Bhattacharya Bhabatarak
Chakrabarti Pinakpani
Das Gupta Sujoy K.
Roy Siddhartha
Saha Abhik
Council of Scientific and Industrial Research
Mondesi Robert B
Ogunbiyi Oluwatosin
Summa, Additon & Ashe, P.A.
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