Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1992-07-28
1994-03-08
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4352401, 4352402, 435243, 4352523, 43525233, 4353201, 43525411, 43525421, 530350, 536 234, 935 44, 935 47, C12N 100, C12N 510, C12N 1562, C12N 1563, C07K 1300
Patent
active
052926460
ABSTRACT:
This invention provides a fusion molecule comprising a DNA sequence encoding a thioredoxin-like protein fused to the DNA sequence encoding a selected heterologous peptide or protein. The peptide or protein may be fused to the amino terminus of the thioredoxin-like molecule, the carboxyl terminus of the thioredoxin-like molecule, or within the thioredoxin-like molecule, for example at the active-site loop of said molecule. Expression of this fusion molecule under the control of a regulatory sequence capable of directing its expression in a desired host cell, produces high levels of stable and soluble fusion protein. The fusion protein, located in the bacterial cytoplasm, may be selectively released from the cell by osmotic shock or freeze/thaw procedures. It may be optionally cleaved to liberate the soluble, correctly folded heterologous protein from the thioredoxin-like portion.
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LaVallie Edward R.
McCoy John
Bugaisky G. E.
DesRosier Thomas J.
Eisen Bruce M.
Genetics Institute Inc.
Meinert Maureen C.
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