Pentaclethera macroloba protein having insecticidal properties

Details Pentaclethera macroloba protein having insecticidal properties Pentaclethera macroloba protein having insecticidal properties

1995-11-20

1997-09-30

Tsang, Cecilia J.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

514 10, 530350, 530370, 530379, A61K 3804, A61K 3800, A61K 3578, C07K 100

Patent

active

056726805

ABSTRACT:
The invention comprises purified trypsin inhibitors extracted from the seeds of Pentaclethra macroloba. The crude extract was found to comprise two molecular weight ranges of 38-35 and 6-9 kDa which contain the active compounds. Within each molecular weight range, the trypsin inhibitors were found to exist in two isoforms. The lower molecular weight inhibitor exhibits surprising stability and activity after heating in aqueous solution at approximately 100.degree. C. for 30 minutes.

REFERENCES:
Kngsley, Mbajunwa Obinna; "Effect of Processing on Some Antinutritive and Toxic Components and on the Nutritional Composition of the African Oil Bean Seed "(Pentaclethra macrophylla Benth); J Sci Food Agric 1995, 68, 153-156.
Negreiros, Andre N. Monte, et al.; "The Complete Amino Acid Sequence of the Major Kunitz Trypsin Inhibitor from the Seeds of Prosopsis Juliflora"Phytochemistry; vol. 30, No. 9 pp. 2829-2833 (1991).
Wu, Han-Chung, et al.; "The Complete Amino Acid Sequence of a Kunitz Family Trypsin Inhibitor from Seeds of Acacia confusa"; J. Biochem. 113, 258-263 (1993).
Negreiros,Andre N. Monte, et al.; "The Complete Amino Acid Sequence of the major Kunitz Trypsin Inhibitor from the Seeds of Prosopsis Juliflora"Phytochemistry; vol. 30, No. 9 pp. 2829-2833 (1991).
Yamamoto, Masashi, et al.; "Amino Acid Sequence of Two Trypsin Inhibitors from Winged Bean Seeds" (Psophocarpus tetragonolobus (L) DC.); J. Biochem. 94, 849-863 (1983).
Kim, Seung-Ho, et al.; "Comparative Study on Amino Acid Sequences of Kunitz-Type Soybean Trypsin Inhibitors, Ti.sup.a , Ti.sup.b , and Ti.sup.c "; J. Biochem. 98, 435-448 (1985).
Richardson, M., et al.; "The Amino Acid Sequence and Reactive (Inhibitory) Site of the Major Trypsin Isoinhibitor (DE5) Isolated from Seeds of the Brazilian Carolina Tree (Adenanthera pavonina L.)"; Biocimica of Biophysica Acta 872 134-140 (1986).
Ryan, Clarence A.; "Protease Inhibitors in Plants: Genes for Improving Defenses Against Insects and Pathogens", Annu. Rev. Phytopathol. 28:425-429 (1990).
Chun, et al. Pentaclethra macroloba Seed Effect on Larval Gowth, Cell Viability, and Midgut Enzyme Acitivty of Helicoverpa zea (Lepidoptera: Noctuidae), Journal of Economic Entomology, vol. 87, No. 6, pp. 11754-1760. Dec. 1994.

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