PCV2 immunogenic compositions and methods of producing such...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

Reexamination Certificate

active

07910306

ABSTRACT:
An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

REFERENCES:
patent: 5322774 (1994-06-01), Peakman
patent: 5565205 (1996-10-01), Petersen
patent: 5580557 (1996-12-01), Kramer
patent: 5733555 (1998-03-01), Chu
patent: 5885823 (1999-03-01), Knittel
patent: 5925359 (1999-07-01), Van Woensel
patent: 5968525 (1999-10-01), Fitzgerald
patent: 6217883 (2001-04-01), Allan
patent: 6287856 (2001-09-01), Poet
patent: 6294176 (2001-09-01), Cochran
patent: 6368601 (2002-04-01), Allan
patent: 6391314 (2002-05-01), Allan
patent: 6497883 (2002-12-01), Bublot et al.
patent: 6660272 (2003-12-01), Allan
patent: 6703023 (2004-03-01), Jestin et al.
patent: 6794163 (2004-09-01), Liu
patent: 6841364 (2005-01-01), Yuan
patent: 6846477 (2005-01-01), Keich
patent: 6943152 (2005-09-01), Audonnet
patent: 6953581 (2005-10-01), Allan
patent: 7018638 (2006-03-01), Chu
patent: 7109025 (2006-09-01), Eloit et al.
patent: 7122192 (2006-10-01), Allan
patent: 7144698 (2006-12-01), Wang
patent: 7148015 (2006-12-01), Jestin
patent: 7169394 (2007-01-01), Chu
patent: 7172899 (2007-02-01), Liu
patent: 7179472 (2007-02-01), Jestin
patent: 7192594 (2007-03-01), Haines
patent: 7211379 (2007-05-01), Ellis
patent: 7223207 (2007-05-01), Basyuk
patent: 7223407 (2007-05-01), Jestin et al.
patent: 7223594 (2007-05-01), Jestin
patent: 7244433 (2007-07-01), Jestin
patent: 7258865 (2007-08-01), Jestin
patent: 7261898 (2007-08-01), Jestin
patent: 7273617 (2007-09-01), Yuan
patent: 7276353 (2007-10-01), Meng
patent: 7279166 (2007-10-01), Meng
patent: 7297537 (2007-11-01), Jestin
patent: 7300785 (2007-11-01), Meerts
patent: 7314628 (2008-01-01), Jestin
patent: 7323330 (2008-01-01), Jestin
patent: 7335361 (2008-02-01), Liao
patent: 7358075 (2008-04-01), Allibert
patent: 7368117 (2008-05-01), Fetzer
patent: 7371395 (2008-05-01), Parisot
patent: 7390494 (2008-06-01), Jestin
patent: 7405075 (2008-07-01), Jestin
patent: 7407803 (2008-08-01), Jestin
patent: 7425444 (2008-09-01), Jestin
patent: 2003/0170270 (2003-09-01), Meng
patent: 2004/0062775 (2004-04-01), Jestin
patent: 2004/0076635 (2004-04-01), Jestin
patent: 2004/0091502 (2004-05-01), Jestin
patent: 2004/0132178 (2004-07-01), Haines
patent: 2004/0161410 (2004-08-01), Jestin
patent: 2004/0253270 (2004-12-01), Meng
patent: 2004/0265848 (2004-12-01), Jestin
patent: 2005/0008651 (2005-01-01), Jestin
patent: 2005/0058653 (2005-03-01), Ellis
patent: 2005/0079185 (2005-04-01), Parisot
patent: 2005/0084497 (2005-04-01), Jestin
patent: 2006/0002952 (2006-01-01), Haines
patent: 2006/0029617 (2006-02-01), Charreyre
patent: 2006/0115489 (2006-06-01), Birkett
patent: 2006/0204522 (2006-09-01), Kroll
patent: 2006/0222659 (2006-10-01), Jestin
patent: 2006/0233831 (2006-10-01), Parisot
patent: 2006/0286123 (2006-12-01), Fetzer
patent: 2008/0181910 (2008-07-01), Roof
patent: 2008/0233147 (2008-09-01), Jestin
patent: 2008/0261887 (2008-10-01), Roof
patent: 2008/0279875 (2008-11-01), Roof
patent: 2008/0279876 (2008-11-01), Roof
patent: 2008/0279889 (2008-11-01), Roof
patent: 1281760 (2003-02-01), None
patent: 1386617 (2004-02-01), None
patent: 89/06972 (1989-08-01), None
patent: 90/07935 (1990-07-01), None
patent: 91/18627 (1991-12-01), None
patent: 92/03157 (1992-03-01), None
patent: 93/16726 (1993-09-01), None
patent: 95/30437 (1995-11-01), None
patent: 99/18214 (1999-04-01), None
patent: 99/29717 (1999-06-01), None
patent: 99/29871 (1999-06-01), None
patent: 00/47756 (2000-08-01), None
patent: 00/77188 (2000-12-01), None
patent: 01/14550 (2001-03-01), None
patent: 01/16330 (2001-03-01), None
patent: 01/17551 (2001-03-01), None
patent: 01/17556 (2001-03-01), None
patent: 02/49666 (2002-06-01), None
patent: 03/003941 (2003-01-01), None
patent: 2004/058142 (2004-07-01), None
patent: 2004/069184 (2004-08-01), None
patent: 2005/009462 (2005-02-01), None
patent: 2006/072065 (2006-07-01), None
patent: 2006/113372 (2006-10-01), None
patent: 2006/113373 (2006-10-01), None
patent: 2007/028823 (2007-03-01), None
patent: 2007/076520 (2007-07-01), None
Kim et al, The Veterinary Journal, 2003, vol. 166, pp. 251-256.
Yang, Zong-zhao, A survey on porcine circovirus type 2 infection and phylogenetic analysis of its ORF2 gene in Hangzhou, Zhejiang Province, China, J Zhejiang Univ Sci B. Feb. 2008; 9(2): 148-153.
Does stress-free livestock mean safer food!, http://www.foodnavigator.com/Financial-Industry/Does-stress-free-livestock-mean-safer-food, Jun. 4, 2004.
Vaccination Guidelines For Swine VIDO Swine Technical Group—Linking knowledge to practical solutions, Vaccination Guidelines, www.vido.org, Jun. 2004.
Albina et al., An Experimental Model for Post-weaning Multisystemic Wasting Syndrome (PMWS) in Growing Piglets, J. Comp. Path., 2001, vol. 123, 292-303.
Allan et al., Experimental infection of colostrum deprived piglets with porcine circovirus 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) potentiates PCV2 replication, Arch. Virol., 2000, 145: 2421-2429.
Allan et al., Porcine circoviruses: a review, J. Vet. Diagn. Invest., 2000, 12:3-14.
Allan et al., Reproduction of postweaning multisystemic wasting syndrome in pigs experimentally inoculated with a Swedish porcine circovirus 2 isolate, 2003, 15:553-560.
Bassaganya-Riera et al., Conjugated Linoleic Acid Ameliorates Viral Infectivity in a Pig Model of Virally Induced Immunosuppression, American Society for Nutritional Sciences, 2003, 3204-3214.
Blanchard et al., Protection of swine against post-weaning multisystemic wasting syndrome (PMWS) by porcine circovirus type 2 (PCV2) proteins, Vaccine 21, 2003, 4565-4575.
Boisseson et al., Molecular characterization of Porcine circovirus type 2 isolates from post-weaning multisystemic wasting syndrome-affected and non-affected pigs, J Gen Virol, 2004, 85, 293-304.
Bolin et al., Postweaning multisystemic wasting syndrome induced after experimental inoculation of cesarean-derived, colostrum-deprived piglets with type 2 porcine circovirus, J Vet Diagn Invest, 2001, 13:185-194.
Chae, C., Postweaning multisystemic wasting syndrome: a review of aetiology, diagnosis and pathology, The Veterinary Journal, 2004, 168:41-49.
Cheung et al., Kinetics of porcine circovirus type 2 replication, Arch Virol, 2002, 147:43-58.
Darwich et al., Cytokine profiles of peripheral blood mononuclear cells from pigs with postweaning multisystemic wasting syndrome in response to mitogen, superantigen or recall viral antigens, J Gen Virol, 2003, 84, 3453-3457.
Fenaux et al., A Chimeric Porcine Circovirus (PCV) with the Immunogenic Capsid Gene of the Pathogenic PCV Type 2 (PCV2) Clones into the Genomic Backbone of the Nonpathogenic PCV1 Induces Protective Immunity against PCV2 Infection in Pigs, J Virol, Jun. 2004, vol. 78, No. 12, 6297-6303.
GenBank Accession No. AAC61738, Version AAC61738.1 GI:3661517, Sep. 29, 1998.
Allan et al., Guest Editorial, PCV-2 infection in swine; more than just postweaning multisystemic wasting syndrome, The Veterinary Journal, 2003, 166:222-223.
Inumaru et al., Expression of biologically active recombinant porcine GM-CSF by baculovirus gene expression system, Immunology and Cell Biology, 1998, 76:195-201.
Ju et al., Immunogenicity of a recombinant pseudorabies virus expressing ORF1-ORF2 fusion protein of porcine circovirus type 2, Veterinary Microbiology, 2005, 109:179-190.
Kim et al., Association of porcine circovirus 2 with porcine respiratory disease complex, The Veterinary Journal, 2003, 1

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

PCV2 immunogenic compositions and methods of producing such... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with PCV2 immunogenic compositions and methods of producing such..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and PCV2 immunogenic compositions and methods of producing such... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2724543

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.