PCR primers for detection of plant pathogenic species and subspe

Details PCR primers for detection of plant pathogenic species and subspe PCR primers for detection of plant pathogenic species and subspe

1999-09-10

2000-11-14

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 4351723, 4351773, 435317, 536 27, 536 28, 536 29, 935 16, 935 17, 935 18, C12Q 168

Patent

active

061468347

ABSTRACT:
We sequenced a 625 and 617 bp fragment of the inner spacer region of 16S-23S rDNA of a strain of Acidovorax avenae representing pathogens from several hosts, including foxtail, oats, corn, rice, millet, sugarcane, orchid, and watermelon and a strain of A. avenae subsp. citrulli pathogenic only to watermelon, respectively, for the purpose of designing PCR primers for their identification. These plant pathogens were previously considered as non-fluorescent pseudomonads and have been recently reclassified as Acidovorax avenae subsp. avenae, A. avenae subsp. cattleyae, and A. avenae subsp. citrulli. Several sets of primers were designed. Primers identified by SEQ ID NO:1 and SEQ ID NO:2 of subsp. avenae reacted with all strains of A. avenae subsp. avenae (previously named P. avenae or P. alboprecipitans) originating from foxtail, oats, corn, rice, sugarcane, and millet, A. avenae subsp. cattleyae from orchid, and A. avenae subsp. citrulli (previously named P. pseudoalcaligenes subsp. citrulli) from watermelon. Primers identified by SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6 of subsp. citrulli reacted with all strains of A. avenae subsp. citrulli, but not with any other strain of subsp. avenae. None of fifty-three other bacteria tested reacted with either set of primers. The citrulli- specific primers should prove especially useful for specific, sensitive, and rapid detection of this serious seedborne pathogen of watermelon seeds.

REFERENCES:
Gurtler et al., Microbiology, vol. 141, pp. 1255-1265, 1995.
Harvey et al., J. Bacteriology, vol. 170, pp. 1235-1238, 1988.
Islam et al., J. Clinical Microbiology, vol. 30, pp. 2801-2806, 1992.
Judd et al., Applied Environmental Microbiology, vol. 59, pp. 1702-1707, 1993.
Katz et al., Am. J. Vet. Res., vol. 54, pp. 2021-2026, 1993.
Loughney et al., Nuc. Acids Res., vol. 10, pp. 1607-1624, 1982.
Luneberg et al., J. Clinical Microbiology, vol. 31, pp. 1088-1094, 1993.
Prosen et al., Phytopathology, vol. 83, pp. 965-970, 1993.
Schaad et al., Int. J. Syst. Bacteriol., vol. 28, pp. 117-125, 1978.
Schaad et al., Phytopathology, vol. 85, pp. 243-248, 1995.
Welsh et al., Nucleic Acids Res., vol. 18. pp. 7213-7218, 1990.
Willems et al., Int. J. Syst. Bacteriol., vol. 42, pp. 107-119, 1992.
Zavaleta et al., Microbiology, vol. 142, pp. 2105-2114, 1996.
Minsavage et al., Ann. Mtg. Amer. Phytopathology, Abstract 379, 1995.
Zeigler et al., Intl. Rice Res. Newsletter, vol. 14(1), pp. 27-28, 1989.
Song et al., Phytopathology, vol. 87(6), pp. 92, 1997.
Kim et al., Seed Science & Technology, vol. 24, pp. 571-580, 1996.
Shakya, D.D., Korean J. Plant Pathology(Abstract), vol. 3, p. 300, 1987.

Affiliated with

Also associated with

Rating

PCR primers for detection of plant pathogenic species and subspe does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with PCR primers for detection of plant pathogenic species and subspe, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and PCR primers for detection of plant pathogenic species and subspe will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2063735