Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-08-29
2006-08-29
Goldberg, Jeanine A. (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S023100, C536S024300, C536S024320
Reexamination Certificate
active
07097975
ABSTRACT:
Soybean rust occurs in many countries throughout Asia, Australia, Africa, and South America. The causal agents of soybean rust are two closely related fungi,Phakopsora pachyrhiziandP. meibomiae, which are differentiated based upon morphological characteristics of the telia. Determination of the nucleotide sequence of the internal transcribed spacer (ITS) region revealed greater than 99%/95% nucleotide sequence similarity among isolates of eitherP. pachyrhiziorP. meibomiae, but only 80% sequence similarity between the two species. Utilizing differences within the ITS region, four sets of PCR primers were designed specifically forP. pachyrhizi, and two sets of PCR primers were made specific toP. meibomiae. Classical and real-time fluorescent PCR assays were developed to identify and differentiate betweenP. pachyrhiziandP. meibomiae.
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Frederick, R., et al., “Detection and Discrimination of the Soybean Rust PathogensPhakopsora pachyrhiziandPhakopsora meibomiaeusing PCR”, (Abstract), Phytopathology, vol. 90, (6), (Supplement), p. 525, 2000.
Fado John D.
Goldberg Jeanine A.
Rabin Evelyn M.
The United States of America as represented by the Secretary of
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