PCR identification and quantification of important Candida speci

Details PCR identification and quantification of important Candida speci PCR identification and quantification of important Candida speci

1996-03-29

2000-01-25

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 2431, 435921, C12Q 168

Patent

active

060176997

ABSTRACT:
The subject invention relates to a set of DNA primers which, when utilized in conjunction with the polymerase chain reaction (PCR) assay, can amplify and speciate DNA from five medically important Candida species. Furthermore, the PCR amplified products, generated by the primers, can also be used to create species specific probes which can also detect and confirm the five species of Candida. Thus, the present invention allows for early diagnosis and treatment of an infection. The assay is useful in the context of monitoring antifungal treatment regimens, screening potential antifungal agents, and similar applications requiring quantitative determinations.

REFERENCES:
patent: 5426026 (1995-06-01), Jordan
patent: 5426027 (1995-06-01), Lott et al.

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