PCR-based strategy of constructing chimeric DNA molecules

Details PCR-based strategy of constructing chimeric DNA molecules PCR-based strategy of constructing chimeric DNA molecules

1991-08-09

1994-01-18

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 912, 435 9141, 935 23, C12N 1510, C12P 1934

Patent

active

052799522

ABSTRACT:
The invention relates to methods for the use of the polymerase chain reaction to amplify a segment of a cloned gene of interest in such a way as to allow a simplified introduction of alterations such as deletions, insertions, repetitions (both direct and inverted) and substitutions into the cloned gene in a specific and precise manner. The unique, amplified segment of the cloned gene so amplified is a common substrate for each of the different approaches to introducing the various alterations into the gene. Choice of the primer sites within the amplified segment coupled with choice of the orientation of the molecule once ligated to itself results in the various resulting embodiments of the invention.

REFERENCES:
Jones et al., Diotechniques, vol. 8, 1990, pp. 178-183.
Higuchi, "Using PCR to Engineer DNA", in PCR Technology, 1990, M. Stockton Press, N.Y., pp. 61-70.
Mole et al., Using the Polymerase Chain Reaction to Modify Expression Plasmids for Epitope Mapping, Nucleic Acids Research (1989) vol. 17, No. 8, p. 3319.
Silver and Keerikatte, Novel Use of Polymerase Chain Reaction to Amplify Cellular DNA Adjacent to an Integrated Provirus, Journal of Virology (1989) 63:1924-1928.
Triglia et al., A Procedure for In Vitro Amplification of DNA Segments that Lie Outside the Boundaries of Known Sequences, Nucleic Acids Research (1988) vol. 16, No. 16, p. 8186.
Wilson et al., Expression Vector pT7: TKII for the Synthesis of Authentic Biologically Active RNA Encoding Vaccinia Virus Thymidine Kinase, 1989, Oregon Agricultural Experiment Station Technical Paper No. 8734, pp. 69-78.
Orlandi et al., Cloning Immunoglobulin Variable Domains for Expression by the Polymerase Chain Reaction, Proc. Natl. Acad. Sci., (1989) pp. 3833-3837.
Horton et al., Engineering Hybrid Genes Without the Use of Restriction Enzymes: Gene Splicing by Overlap Extension, Gene (1989) 77:61-68.
Abbot et al., The Gene for Human Complement Component C9 Mapped to Chromosome 5 by Polymerase Chain Reaction, Genomics 4, (1989) pp. 606-609.
Pratt et al., Ig V Region Gene Expression in Small Lymphocytic Lymphoma with Little or No Somatic Hypermutation, Journal of Immunology, (Jul. 1989), vol. 143, No. 2, pp. 699-705.
Carroll et al., Absence of Ig V Region Gene Somatic Hypermutation in Advanced Burkitt's Lymphoma, Journal of Immunology (Jul. 1989) vol. 143, No. 2, pp. 692-698.
Oste et al., Polymerase Chain Reaction, BioTechniques (1988) vol. 6, No. 2, pp. 162-167.
Vallette et al., Construction of Mutant and Chimeric Genes Using the Polymerase Chain Reaction, Nucleic Acids Research (1989) vol. 17, pp. 723-733.
Syvanen et al., Quantification of Polymerase Chain Reaction Products by Affinity-Based Hybrid Collection, Nucleic Acids Research (1988) 16:11327-11338.
Shuldiner et al., Hybrid DNA Artifact from PCR of Closely Related Target Sequences, Nucleic Acids Research (1989) 11:4409.
Morgan et al., Polymerase Chain Reaction for Detection of Residual Leukaemia The Lancet (1989) pp. 928-929.

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