PCR-based cDNA subtractive cloning method

Details PCR-based cDNA subtractive cloning method PCR-based cDNA subtractive cloning method

1997-05-23

1998-12-29

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 935 76, 935 77, 935 78, 536 244, C12Q 168, C12P 1934, C12N 1500, C07H 2104

Patent

active

058539913

ABSTRACT:
A process is described in which PCR-based cDNA libraries with anchored ends are made, a PCR-based cDNA subtracted (PCSUB) library is produced therefrom, and the ends of a cDNA clone are isolated, via PCR, from an anchored-ends library.

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