PCR-based assay for Mycoplasma hyopneumoniae

Details PCR-based assay for Mycoplasma hyopneumoniae PCR-based assay for Mycoplasma hyopneumoniae

1993-05-18

1998-01-27

Arthur, Lisa B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 2433, 536 2432, C12Q 168, C12P 1934, C07H 2104

Patent

active

057120900

ABSTRACT:
Disclosed herein is a PCR-based assay for Mycoplasma hyopneumoniae, a species-specific primer pair for use in the assay, and a related diagnostic kit. The primer pair is made up of an oligonucleotide having the nucleotide sequence 5'-AAGTTCATTCGCGCTAGCCC-3' and an oligonucleotide having the nucleotide sequence 5'-GCTCCTACTCCATATTGCCC-3'. Preferably, the kit contains an oligonucleotide probe having the sequence 5'-GGTAGCCCTTCCTTTGAGGT-3'.

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