PCR amplification of mRNA

Details PCR amplification of mRNA PCR amplification of mRNA

1995-08-04

1998-09-29

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

935 77, 935 78, C12P 1934

Patent

active

058144897

ABSTRACT:
A method for producing a contaminating DNA free single stranded PCR amplification product of mRNA is disclosed. The mRNA amplification product is of lower molecular weight and is readily separated, e.g., by gel electrophoresis, from any amplified cellular DNA contaminant.

REFERENCES:
Sancho-Tello et al., Endocrinology 130(3):1359-1364 (1992).
Watson et al., Molecular Biology of the Gene 4th Edn: The Benjamin Kummings Publishing Company, Inc., Menlo Park, CA. 1987 pp. 626-629.
Scanlon, et al., "Molecular Baiss of Cisplatin Resistance in Human Carcinomas: Model Systems and Patients" Anticancer Research 9:1301-1312 (1989).
Scanlon, et al., "Utility of the Polymerase Chain Reaction in Detection of Gene Experssion in Drug-Resistant Human Tumors" Journal of Clinical Laboratory Analysis 3:323-329 (1989).
Kashani-Sabet, et al., "Detection of Drug Resistance in Human Tumors by in Vitro Enzymatic Amplification" Cancer Research 48:5775-5778 (1988).

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