Pathogen detection using coherent anti-stokes Raman...

Optics: measuring and testing – By dispersed light spectroscopy – With raman type light scattering

Reexamination Certificate

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C356S036000

Reexamination Certificate

active

07542137

ABSTRACT:
The invention provides a system and method for automatic real-time monitoring for the presence of a pathogen in water using coherent anti-stokes Raman scattering (CARS) microscopy. Water sample trapped in a trapping medium is provided to a CARS imager. CARS images are provided to a processor for automatic analyzing for the presence of image artifacts having pre-determined features characteristic to the pathogen. If a match is found, a CARS spectrum is taken and compared to a stored library of reference pathogen-specific spectra for pathogen identification. The system enables automatic pathogen detection in flowing water in real time.

REFERENCES:
patent: 6950184 (2005-09-01), Stewart et al.
Grow et al, New Biochip Technology for Label-Free Detection of Pathogens and Their Toxins, Journal of Microbiological Methods, vol. 53, Issue 2, May 2003, pp. 221-233.
“Broadly tunable dual-wavelength light source for coherent anti-stokes raman scattering microscopy”, Ganikhanav et al, Optics Letters, vol. 31, No. 9, May 1, 2006.
“Optimization of coherent anti-stokes raman scattering microscopy using photonic crystal fiber”, Murugkar et al (to be published in Proceedings of SPIE 2007).
“A fast method for detecting Cryptosporidium parvum oocysts in real world samples”, Stewart et al, Advanced Biomedical and Clinical Diagnostic Systems III, SPIE, vol. 5692 (SPIE Bellingham, WA, 2005).
“Multi-focus coherent anti-stokes raman scattering microscopy”, Hashimoto et al, Microsc. Microanal 9 (Suppl 2), 2003, Copyright 2003 Microscopy Society of America.

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