Pathogen and stress-responsive promoter for gene expression

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers pathogen or pest resistance

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435 691, 4353201, 435418, 435419, 435468, 536 236, 536 241, 800287, 800301, C12N 504, C12N 1529, C12N 1582, A01H 500

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active

060721030

ABSTRACT:
Nucleic acid sequences and methods for their use are provided for obtaining plants with inducible phenotypes, including novel or enhanced resistance to infection and stress. Novel nucleic acid constructs are provided, which include a transcriptional initiation region from a pathogen inducible-rapid response grape stilbene synthase gene, a DNA sequence of interest and a transcriptional termination region. The constructs are used to prepare expression cassettes which may then be introduced into plants and portions thereof for modulation of expression of endogenous products as well as production of exogenous products in the plant.

REFERENCES:
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Hain, R. et al., "Disease Resistance Results from Foreign Phytoalexin Expression in a Novel Plant" Nature, vol. 361, Jan. 1993, pp:153-156.
Stark Lorenzen P., et al., "Transfer of a Grapevine Stilbene Synthase Gene to Rice (Oryza sativa L.)" Plant Cell Reports, vol. 19, Jul. 1997, pp: 668-673.
Schubert, R., et al., "An ozone-responsive region of the grapevine resveratrol synthase promoter differs from the basal pathogen-- responsive sequence" Plant Molecular Biology 34:417-426 (1997).
Melchior, F., et al., "Coordinate- and elicitor- Dependent Expression of Stilbene synthase and Phenylalaine Ammonia-Lyase Genes in Vitis cv. Optima" Archives of Biochemistry and Biophysics vol. 288:2 pp. 552-557 (1991).
Wiese, W., et al., "Structural organization and differential expression of three stilbene synthase genes located on a 13 kb grapevine DNA fragment" Plant Molecular Biology 26:667-677, (1994).

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