Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is a biological cell or cell fragment
Patent
1987-01-07
1990-01-02
Benson, Robert
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is a biological cell or cell fragment
436520, 436522, 436528, 436533, 436534, 436535, 436546, 436800, 436805, 436808, 436809, 436821, 436823, 436829, 428402, G01N 33533
Patent
active
048913243
ABSTRACT:
Assay methods are provided for determining an analyte in a sample suspected of containing the analyte. The method is carried out using a composition that includes a conjugate of a first sbp member with a particle. A luminescer is reversibly associated with a nonaqueous phase of the particle. Where the first sbp member is not complementary to the analyte, a second sbp member that is capable of binding to the first sbp member is employed. Unbound conjugate is separated from conjugate that is bound to the analyte or to the second sbp member. A reagent for enhancing the detectability of the luminescer is added and the light emission of the luminescer acted on by the reagent is measured.
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Kirakossian Hrair
Pease John
Ullman Edwin F.
Weng Litai
Barrett Carole F.
Benson Robert
Leitereg Theodore J.
Swiss Gerald F.
Syntex (U.S.A.) Inc.
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