Particle washing and separation method

Details Particle washing and separation method Particle washing and separation method

1985-10-30

1987-10-06

Fisher, Richard V.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Particle count or volume standard or control

210789, 210927, 436 16, 494 16, G01N 100, B01D 3302, B04B 502

Patent

active

046983112

ABSTRACT:
A device is described for preparing a standardized concentration of washed particles suspended in a fluid. The preferred method is directed to the preparation from whole blood specimens of a standardized concentration of washed red cells in isotonic solution using a device comprising a test tube formed at the bottom end with a nipple-like receptacle having a predetermined volumetric capacity. Whole blood is mixed with isotonic solution in the device which is then centrifuged causing the red cells to be first washed and then packed into the receptacle. The liquid contents of the device are then decanted without disturbing the packed red cells. Thereafter, the red cells are reconstituted to the desired concentration by adding isotonic solution to a predetermined level in the tube and mixing the cells into the isotonic solution.

REFERENCES:
patent: 1907605 (1933-05-01), Stigen
patent: 3406121 (1968-10-01), Jones
patent: 4106907 (1978-08-01), Charlton et al.
patent: 4308232 (1981-12-01), Crouther et al.
patent: 4486315 (1984-12-01), Teipel

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