Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-04-02
1995-11-28
Fleisher, Mindy B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4351723, 4352402, C12Q 168, C12N 1590, C12N 516, C12N 522
Patent
active
054707080
ABSTRACT:
A method of genetically transforming mammalian unattached cells is disclosed. The method begins by preparing copies of a nucleic acid construct and coating these copies onto biologically inert carrier particles. Mammalian unattached cells are isolated in a liquid suspension. The cell suspension is placed on a target surface, wherein the liquid is spread to a thin film on the target surface. In an alternative embodiment of the present invention, the liquid is spread onto a porous surface. The cells are bombarded with the construct-coated particles in such a fashion that some particles lodge in the interior of at least some of the cells. The existence and expression of the construct within the cell is verified.
REFERENCES:
patent: 4945050 (1990-07-01), Sanford et al.
patent: 5015580 (1991-05-01), Christon et al.
Yang et al., PNAS, vol. 87, 1990, pp. 9568-9572.
Friedmann, T., "Progress Toward Human Gene Therapy," 244 Science 1275-1281 (1989).
Johnston, S. A., "Biolistic Transformation: Microbes To Mice," 34 Nature 776-777 (Aug. 1990).
Johnston, S. A., "In-Situ Introduction of Genes Into Tissues of the Living Mouse," Symposium on Particle Bombardment-Mediated Transformation of Plant and Animal Systems, Sponsored by Agracetus, Inc., Mar. 14-15, 1991.
Sanford, et al., "Delivery of Substances Into Cells and Tissues Using a Particle-Bombardment Process," 5 Particulate Science and Technology 27-37 (1987).
Sanford, J. C. "Recent Advances in Biolistic Technology," Symposium on Particle Bombardment-Mediated Transformation of Plant and Animal Systems, Sponsored by Agracetus, Inc., Mar. 14-15, 1991.
Sanford, J. C., "The Biolistic Process," 6 TIBTECH 299-302.
Yang, N. S., et al., "In Vivo and In Vitro Gene Transfer to Mammalian Somatic Cells by Particle Bombardment," Symposium on Particle Bombardment-Mediated Transformation of Plant and Animal Systems, Sponsored by Agracetus, Inc., March 14-15, 1991.
Zelenin, A. V., et al., "Genetic Transformation of Mouse Cultures Cells with Help of High-Velocity Mechanical DNA Injection", 244 FEBS Letters 65-67 (1989).
Benner, R., et al., "Antibody Formation in Bone Marrow," In Immunological Methods vol. 2, 248-250 (1981).
Human Growth Hormone Transient Gene Expression Assay System, Nicholas Institute Diagnostics.
MacGregor, et al., "histochemical Staining of Clonal Mammalian Cell Lines Expressing E. coli, Beta-glactosidase Indicates Heterogenous Expression of the Bacterial Gene," Somatic Cell and Molecular Genetics 253-265 (1987).
Quesenberry, P. J., "Hemopoietic Stem Cells, Progenitor Cells, and Growth Factors," In Hematology (1990).
Selected Methods in Cellular Immunology, 11, 22, 153, 205-208.
Wood, K. V., "Firefly Luciferase: A New Tool for Molecular Biologists," Promega Notes (Dec. 1990).
Miller, A. D., "Progress Toward Human Gene Therapy," 76 Blood 271-278 (1990).
Burkholder Joseph K.
Fuller Deborah L.
Swain William F.
Yang Ning-Sun
Fleisher Mindy B.
Ketter James
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