Optics: measuring and testing – Blood analysis
Patent
1984-05-04
1986-08-19
McGraw, Vincent P.
Optics: measuring and testing
Blood analysis
250574, 356338, 422 67, G01N 3349, G01N 114
Patent
active
046066314
ABSTRACT:
A first syringe pump feeds a sample solution such as, for example, a diluted blood into an inner tube of a flow cell, and a second syringe pump feeds a sheath solution such as, for example, a physiological saline solution into an outer tube of the flow cell. Respective first and second plungers of the first and second syringe pumps are fixed onto a connecting plate. The connecting plate is supported by a pair of guide rods so that it may be moved in line with the moving direction of the plungers. Into the connecting plate is screwed an externally threaded rod, which is driven by a motor to rotate in the normal and reverse directions about its axis. By the normal and reverse rotations of the threaded rod, the connecting plate is reciprocally moved, whereby the first and second plungers are moved at the same speed. By the movement of the first and second plungers at the same speed, the sample solution and the sheath solution are respectively fed into the flow cell at the same rate. Thus, in the flow cell, two flows of solution (water sheath), consisting respectively of the sample solution and the sheath solution existing around the sample solution, are formed. Laser beams are irradiated onto the flow of sample solution of the water sheath passing through a small-diameter portion of the flow cell, whereby the laser beams scattered by grains (blood corpuscles) in the sample solution are sensed by a beam sensor.
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The Review of Scientific Instruments; vol. 43, No. 3, Mar. 1972, pp. 404-409, "Fluorescence Activated Cell Sorting", L. A. Herzenberg et al.
Anno Gousuke
Suzuki Yoshinori
Kabushiki Kaisha Toshiba
McGraw Vincent P.
Thompson, III Robert
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