Particle based homogeneous assays using capillary...

Details Particle based homogeneous assays using capillary... Particle based homogeneous assays using capillary...

2007-02-20

2007-02-20

Le, Long V. (Department: 1641)

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

C435S007100, C435S007920, C436S172000, C436S524000, C204S451000

Reexamination Certificate

active

09947990

ABSTRACT:
The invention provides highly sensitive and rapid homogeneous assays which employ particle-enhanced assay formats in concert with capillary electrophoresis and laser-induced fluorescence (LIF) detection to determine the concentration of an analyte of interest in a sample. Such a determination is made by measuring fluorescent signal(s) (i.e., an electropherogram) produced upon LIF of species present in the reaction mixture that are capable of producing such signals. The method of this invention produces simplified electropherograms by reducing the number of signals that must be separated and subsequently measured, and therefore increases the accuracy of the detection and/or quantification of target analyte concentration in a sample.

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Wang, et al., “Noncompetitive immunoassays using protein G affinity capillary chromatography and capillary electrophoresis with laser-induced fluorescence detection”, Journal of Chromatography, Elsevier Science Publishers B.V. Amsterdam, NL, vol. 848, No. 1-2, Jul. 2, 1999, pp. 139-148, XP004184207.

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