Chemistry: analytical and immunological testing – Automated chemical analysis – Utilizing a centrifuge or compartmented rotor
Patent
1996-03-04
1998-07-21
Le, Long V.
Chemistry: analytical and immunological testing
Automated chemical analysis
Utilizing a centrifuge or compartmented rotor
436165, 436177, 436180, 435 71, 422 72, 422 73, 422100, 422101, 210789, G01N 2107
Patent
active
057834466
ABSTRACT:
Methods and cartridges are provided for the detection of analytes in a sample by using fluid velocity gradients. In the subject methods, the assay medium is prepared by combining sample suspected of containing analyte with a reagent composition which includes at least one of particles and specific binding pair members ("sbpm's "). A circumferential fluid velocity gradient and vertical fluid velocity gradient are applied to the assay medium to affect the formation of agglutinates. The sizes of the resulting agglutinates are related to the velocity gradients. The level of agglutination is detected to determine analyte presence in the sample. In general, a cartridge for employing the assay comprises two spaced apart parallel plates defining an assay area and trough area there between. A sample port and optically clear window are provided. At least one of the plates is connected to a rotational driver to rotate the plate in a direction counter to the position of the other plate.
REFERENCES:
patent: 4135819 (1979-01-01), Schmid-Schonbein
patent: 4352557 (1982-10-01), Schmid-Schonbein
patent: 4814282 (1989-03-01), Holen et al.
patent: 4865810 (1989-09-01), Simon
patent: 4916078 (1990-04-01), Klose et al.
patent: 5086002 (1992-02-01), Hillyard et al.
patent: 5173262 (1992-12-01), Burtis et al.
patent: 5242803 (1993-09-01), Burtis et al.
patent: 5310523 (1994-05-01), Smethers et al.
patent: 5523238 (1996-06-01), Varon et al.
Brown, "The Physics of Continuous Flow Centrifugal Cell Separation," Artificial Organs (1989), 13:4-20.
Link and Springer, "Light Scattering From Dilute Polymer Solutions in Shear Flow," Macromolecules (1993), 26:464-471.
Kume et al., "A new Apparatus for Simultaneous Observation of Optical Microscopy and Small-Angle Light Scattering Measurements of Polymers Under Shear Flow," Acta Polymer (1995), 46:79-85.
Ribi Hans O.
Saul Tom
Biocircuits Corporation
Le Long V.
LandOfFree
Particle assay using fluid velocity gradients does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Particle assay using fluid velocity gradients, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Particle assay using fluid velocity gradients will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1646253