Particle agglutination immunoassay with agglutinator for determi

Chemistry: analytical and immunological testing – For preexisting immune complex or auto-immune disease – Rheumatoid factors

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436534, 436805, 436815, 436821, 436825, G01N 3354

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active

044277814

ABSTRACT:
A particle counting assay for haptens (small non-protein monovalent substances having a molecular weight below 1500) comprises mixing a liquid sample (e.g. of human origin) containing the hapten, with finely divided inert particles bearing the same hapten (or a specific analogue thereof), an agglutinator such as RF or C1q, and a measured amount of antibody, which amount is insufficient to cause agglutination of all the particles. The amount of hapten is determinable by measuring the extent of the agglutination.

REFERENCES:
patent: 4162895 (1979-07-01), Cambiaso
patent: 4184847 (1980-01-01), Hallgren
patent: 4279617 (1981-07-01), Masson
patent: 4308026 (1981-12-01), Mochidd
Chemical Abstracts, 95: 146230h, (Oct. 26, 1981).
"Immunoassays for the 80's", A. Voller et al., Eds., pp. 35-41, University Park Press, Baltimore, (1980).

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