Partially purified osteogenic factor and process for preparing s

Details Partially purified osteogenic factor and process for preparing s Partially purified osteogenic factor and process for preparing s

1983-04-12

1984-02-28

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

2601237, 260118, 424 95, 424177, C07G 700

Patent

active

044340940

ABSTRACT:
A process for partially purifying an osteogenic factor from demineralized bone particles in which nonfibrous proteins are extracted from the demineralized bone with a dissociative extractant for nonfibrous proteins, such as urea or guanidine hydrochloride, the extracted nonfibrous proteins are fractionated by anion exchange chromatography using DEAE cellulose at pH 7, the fraction not adsorbed by the DEAE cellulose is further fractionated by cation exchange chromatography using CM cellulose at pH 4.8, the fraction adsorbed by the CM cellulose is eluted therefrom and the partially purified osteogenic factor is recovered from the eluate as a .ltoreq.30,000 dalton protein isolate.

REFERENCES:
patent: 3539549 (1970-11-01), Greenfield
patent: 4294753 (1981-10-01), Urist
Urist et al., Clin. Orthopaedics and Related Research, (1982), 162, 219-232.

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