Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1986-11-26
1988-09-27
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351721, 4351723, 435236, 435253, 935 11, 935 29, 935 72, 935 73, 935 74, C12P 2100, C12N 1500, C12N 704, C12N 120
Patent
active
047741821
ABSTRACT:
Construction of a recombinant bacterial host which is substantially immune to a normally infectious viral agent is disclosed. In one embodiment a foreign recombinant reproducible gene segment is inserted into the bacterial cell. The gene segment is a partially defective variant of a gene segment from the infectious virus of interest. The viral gene segment, in its naturally occurring and fully operational form, normally codes for a non-repressor protein which participates in a multi-protein complex used in the development of the virus. However, the partially defective gene present in the bacterial cell codes for a defective protein that will bind to the remainder of the multi-protein complex with a greater affinity than the virus' fully operational protein. The "new" protein complex will therefore be inactive and will inhibit the ability of the foreign agent to use the multi-protein complex for its development.
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Mays Thomas D.
Wisconsin Alumni Research Foundation
Wiseman Thomas G.
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