Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology
Patent
1998-10-21
2000-06-20
Wortman, Donna C.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of regulating cell metabolism or physiology
C12N 506
Patent
active
060777109
ABSTRACT:
A process of parthenogenic activation of mammalian oocytes which includes increasing intracellular levels of divalent cations in the oocyte; and reducing phosphorylation of cellular proteins in the oocyte. One method of accomplishing this is by introducing Ca.sup.2+ free cation, such as ionomycin, to the oocyte and then preventing phosphorylation of the cellular proteins within the oocyte by adding a serine-threonine kinase inhibitor, such as 6-dimethylaminopurine (DMAP).
REFERENCES:
patent: 4806604 (1989-02-01), Tsien et al.
patent: 4994384 (1991-02-01), Prather et al.
patent: 5096822 (1992-03-01), Rosenkrans
patent: 5180665 (1993-01-01), Holmes
Eusebi et al., Developmental Biology, 96:386-395, 1983.
Rime et al, Developmental Biology, 133:169-179, 1989.
Collas, P. and J.M. Robl, "Factors affecting the efficiency of nuclear transplantation in the rabbit embryo," Biol. Reprod. 43:877-884 (1990).
Cuthbertson, K.S.R., "Parthenogenic activation of Mouse oocytes in vitro with ethanol and benzyl alcohol," J. Exp. Zool. 226:311-314 (1983).
First, N. L. and R. Prather, "Genomic Potential in Mammals," Differentiation 48:1-8 (1991).
First, N. L., Leibfried-Rutledge, M.L., Northey, D.L. and Nuttleman, P.R., "Use of in vitro matured oocytes 24 h of age in bovine nuclear transfer," Theriogenology 37:211 (1992).
Fissore, R.A. and J.M. Robl, "Intracellular Ca2+ response of rabbit oocytes to electrical stimulation," Mol. Reprod. Dev. 32:9-16 (1992).
Fulka, et al., "Effect of 6-dimethylaminopurine on germinal vesicle breakdown of bovine oocytes," Molecular Reproduction and Development 29:379-384 (1991).
Graham, C.F., "Parthenogenic Mouse Blastocysts," Nature 242:475-476 (1970).
Graham, C.F., "The fusion of cells with one and two cell mouse embryos," Wistar Inot. Symp. Monogr. 9:19 (1969).
Graham, C.F., "The production of parthenogenic mammalian embryos and their use in biological research," Biol. Rev. 49:399-422 (1974).
Gray, K.R., K.R. Bondioli and C.L. Betts, "The commercial application of embryo splitting in beef cattle," Theriogenology 35:37-45 (1991).
Kaufman, M.H., "Parthenogenesis: a system facilitating inderstanding of factors that influence early mammalian development," Prog. in Anat. 1:1-34 (1981).
Kline, D. and Kline, J.T., "Repetitive calcium transients and the role of calcium in exocytosis and cell cycle activation in the mouse egg," Dev. Biol. 149:80-89 (1992).
Kubiak, J., "Mouse oocytes gradually develop the capacity for activation during the metaphase II arrest," Dev. Biol. 136:537-545 (1989).
Landa and Hajkova, "Diploidization of bovine oocytes matured in vitro and parthenogenetically activated by electric shock," Folia Biologica 36:145-152 (1990).
Masui, Y. and Markert, C.L., "Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes," J. Exp. Zool. 177:129-146 (1971).
McGrath, J. and Solter, D., "Nuclear Transplantation in the Mouse embryo by microsurgery and cell fusion," Science 220:1301-1302 (1983).
Nagai, T., "Parthenogenic Activation of cattle follicular oocytes in vitro with ethanol," Gamete Res. 16:243-249 (1987).
Nurse, P., "Universal control mechanism regulating onset of M-phase," Nature 344:503-508 (1990).
Onodera, M. and Y. Tsunoda, "Parthenogenic activation of mouse and rabbit eggs by electric stimulation in vitro," Gamete Research 22:277-283 (1989).
Ozil, J.P., "The parthenogenic development of rabbit oocytes after repetitive pulsatile electrical stimulation," Development 109:117-127 (1990).
Rickords, L.F. and White, K.L., "Electrofusion-induced intracellular Ca+2 flux and its effect on murine oocyte activation," Mol. Reprod. Dev. 31:152-159 (1992).
Siracusa, G., Whittingham, D.G., Molinaro, M. and Vivarelli, E., "Parthenogenic activation of mouse oocytes induced by inhibitors of protein synthesis," J. Embryol. Exp. Morph. 43:157-166 (1978).
Sirard, M.A., J.J. Parrish, C.B. Ware, M.L. Leibfried-Rutledge and N.L. First, "The culture of bovine oocytes to obtain developmentally competent embryos," Biol. Reprod. 39:546-552 (1988).
Steinhardt, R.A., Epel, D. and Yanagimachi, R., "Is calcium ionophore a universal activator for unfertilized eggs," Nature 252:41-43 (1974).
Stice, S.L. and Keefer, C. "Improved developmental rates for bovine nucleus transfer embryos using cold shock activated oocytes," Biol. Reprod. 42(Suppl 1):166 (1992).
Stice, S.L. and Robl, J.M., "Activation of mammalian oocytes by a factor obtained from rabbit sperm," Mol. Reprod. Dev. 25:272-280 (1990).
Surani, M.A.H. and Kaufmann, N.H., "Influence of extracellular Ca2+ and Mg2+ ions on the second meiotic division of mouse oocytes: Relevance to obtaining haploid and diploid parthenogenic embryos," Dev. Biol. 59:86-90 (1977).
Susko-Parrish, J.L., Nuttleman, P.R. and Leibfried-Rutledge, M.L., "Effect of bovine oocyte aging in vitro on development," Biol. Reprod. 44(suppl 1):17:156 (abstract) (1991).
Swann, K., "A cytosolic sperm factor stimulates repetitive calcium increases and mimics fertilization in hamster egg," Development 110:1295-1302 (1990).
Tarkowski, A.K., "Induced parthenogenesis in the mouse," The Developmental biology of reproduction (C.L. Market, E.J. Papaconstantinon, eds.), pp. 107-129, New York: Academic Press (1975).
Tombes, R.M., Simerly, C., Borisy, G.G. and Schatten, G., "Meiosis, egg activation and nuclear envelope breakdown are differentially reliant on Ca+2, whereas germinal vesicle breakdown is Ca+2 independent in the mouse oocyte," J. Cell Biol. 117:799-811 (1992).
Ware, C.B., Barnes, F.L., Maik-Laurila, M. and First, N.L., "Age dependence of bovine oocyte activation," Gamete Res., 22:265-275 (1989).
Watanabe, N., Hunt, T., Ikawa, Y. and Sagata N., "Independent inactivation of MPF and cytostatic factor (mos) upon fertilization of Xenopus eggs," Nature 352:247-248 (1991).
Watanabe, N., Hunt, T., Ikawa, Y. and Sagata N., "Specific proteolysis of the c-mos proto-oncogene product by calpain on fertilization of Xenopus eggs," Nature 342:505-510 (1989).
Whitaker, M. and Irvine, F.R. "Inositol 1, 4, 5-trisphophate microinjection activates sea urchin eggs," Nature (London) 312:636-639 (1984).
Yang, X. et al., "Nuclear transfer in rabbits and cattle by electric pulse-induced fusion of blastomeres to enucleated oocytes," Theriogenology 35:298 (abs.) (1991).
Yang, X. et al., "Potential of hypertonic medium treatment for embryo micromanipulation: II. Assessment of nuclear transplantation methodology, isolation, subzona insertion and electrofusion of blastomeres," Mol. Reprod. Dev. 27:118-129 (1990).
Tarkowski, A.K., Witkowska, A. and Nowicka, J., "Experimental parthenogenesis in the mouse, " Nature226:162-165 (1970).
Leibfried-Rutledge M. Lorraine
Northey David L.
Stice Steven L.
Susko-Parrish Joan L.
Brumback Brenda G.
Infigen, Inc.
Wortman Donna C.
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