Parthenogenic oocyte activation

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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424 932, 600 33, C12N 500, A61K 3700, A61B 17425

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054967201

ABSTRACT:
A process of parthenogenic activation of mammalian oocytes which includes increasing intercellular levels of divalent cations in the oocyte; and reducing phosphorylation of cellular proteins in the oocyte. One method of accomplishing this is by introducing Ca.sup.2+ free cation, such as ionomycin, to the oocyte and then preventing phosphorylation of the cellular proteins within the oocyte by adding a serine-threonine kinase inhibitor, such as 6-dimethylaminopurine (DMAP).

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