Parkin substrate and assay

Details Parkin substrate and assay Parkin substrate and assay

2008-05-21

2011-11-22

Liu, Sue (Department: 1653)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Reexamination Certificate

active

08062839

ABSTRACT:
The invention provides in vitro, ex vivo, and in vivo assays for Parkin activity, in which Parkin-mediated ubiquitination of a Sept4 protein is measured. The assays may be used to screen for agents that modulate Parkin protein ligase activity.

REFERENCES:
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patent: 1 416 272 (2004-05-01), None
patent: WO 2008/144736 (2008-11-01), None
Choi et al. “SEPT5—v2 is a parkin-binding protein”, Molecular Brain Research, 2003, 117:179-189.
Hampe et al. “Biochemical analysis of Parkinson's disease-causing variants of Parkin, an E3 ubiquitin-protein ligase with monoubiquitylation capacity”, Human Molecular Genetics, 2006, 15(13):2059-2075.
U.S. Appl. No. 60/939,335, filed May 21, 2007, Johnston.
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Imai et al., “An Unfolded Putative Transmembrane Polypeptide, which can Lead to Endoplasmic Reticulum Stress, is a Substrate of Parkin,”Cell, 105(7):891-902 (2001).
Larisch et al., “A Novel Mitochondrial Septin-Like Protein, ARTS, Mediates Apoptosis Dependent on its P-loop Motif,”Nature Biology, 2:915-921 (2000).
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